Dulbeccos Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies offers a variety of Gibco DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the?media selector tool.

This DMEM / F-12 is modified as follows:

With Without
L-glutamine Sodium Bicarbonate
HEPES ?
Phenol Red ?


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The complete?formulation?is available.

Gibco DMEM / F-12 is a 1:1 mixture of DMEM and Hams F-12. This formulation combines DMEMs high concentrations of glucose, amino acids and vitamins with F-12s wide variety of components.

Product Intended Use
For?in vitro?diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco DMEM / F-12 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco DMEM / F-12 product made in our Scotland facility (42400-010). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10%?Fetal Bovine Serum?(FBS). DMEM / F-12 uses a sodium bicarbonate buffer system (2.438 g / L) and therefore requires a 5-10% CO2?environment to maintain physiological pH.

Powder forms of Gibco cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see?protocol?for details).