Size??100ug
Cat code??200357
Species Cross-reactivity??M
Key Application??WB,ICC,FC
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG1
Molecular Weight??52kDa
Sensitivity??This antibody detects endogenous levels SMAD5 and does not cross-react with related proteins.
Immunogen??Purified recombinant human SMAD5 protein fragments expressed in E.coli.
UniProt Number??Q99717
GeneBank ID??NM_001001420
Subcellular location??Cytoplasm. Nucleus
Formulation??Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername??DWFC; JV5-1; MADH5; DKFZp781C1895; DKFZp781O1323
Gene_symbol??DWFC; JV5-1; MADH5
Summary??SMAD5 is a member of the Mothers Against Dpp (MAD)-related family of proteins. It is a receptor-regulated SMAD (R-SMAD), and acts as an intracellular signal transducer for the transforming growth factor beta superfamily. SMAD5 is activated through serine phosphorylation by BMP (bone morphogenetic proteins) type 1 receptor kinase. It is cytoplasmic in the absence of its ligand and migrates into the nucleus upon phosphorylation and complex formation with SMAD4. Here the SMAD5/SMAD4 complex stimulates the transcription of target genes.
UniPort summary  Function??Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Application Images :??
Western blot detection of SMAD5 antibody in Mouse testis cell lysates using SMAD5 antibody (1:1000 diluted).
Predicted band size:55KDa
Observed band size:55KDa
Immunocytochemistry of HeLa cells using anti- SMAD5 (C-terminus) antibody diluted 1:75.
Flow Cytometry analysis of Jurkat cells stained with SMAD5 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.