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Using Opti-MEM I Medium：
Opti-MEM I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 510% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system：
Opti-MEM I is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Opti-MEM I product made in our Scotland facility (31985-047). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.