Size??100ug
Cat code??200605
Species Cross-reactivity??H,R
Key Application??WB,ICC
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG2b
Molecular Weight??50kDa
Sensitivity??This antibody detects endogenous levels of CSK and does not cross-react with related proteins.
Immunogen??Purified recombinant human CSK protein fragments expressed in E.coli.
UniProt Number??P41240
GeneBank ID??NM_001127190
Subcellular location??Cytoplasm. Cell membrane.
Formulation??Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.2% sodium azide,0.1mg/mlBSA and 50% glycerol.
Altername??C SRC;C SRC kinase;C src Tyrosine Kinase;C-SRC kinase;c-src tyrosine kinase;Csk A;CSK;CSK_HUMAN;CYTOPLASMIC TYROSINE KINASE;EC2.7.10.2;MGC112926;MGC117393;MGC154049;P60 Src;Protein tyrosine kinase CYL;Protein-tyrosine kinase CYL;Proto oncogene tyrosine protein kinase;Tyrosine protein kinase CSK;Tyrosine-protein kinase CSK;zgc:154049.
Gene_symbol??null
Summary??null
UniPort summary  Function??Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.
Application Images :??
Western blot detection of CSK antibody in C6 and Jurkat cell lysates using CSK antibody (1:1000 diluted).Predicted band size:50KDa.Observed band size:50KDa.
Immunocytochemistry staining of C6 cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-CSK antibody (dilution 1:50).