?

• 性質Recombinant
• 來源E. coli
• 氨基酸序列
  • AccessionQ9NT62
  • 種屬Human
  • 序列MQNVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGDHLVHHC PTWQWATGEELKVKAYLPTGKQFLVTKNVPCYKRCKQMEYSDELEAIIEE DDGDGGWVDTYHNTGITGITEAVKEITLENKDNIRLQDCSALCEEEEDED EGEAADMEEYEESGLLETDEATLDTRKIVEACKAKTDAGGEDAILQTRTY DLYITYDKYYQTPRLWLFGYDEQRQPLTVEHMYEDISQDHVKKTVTIENH PHLPPPPMCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAV IPTIEYDYTRHFTM
  • 分子量37 kDa including tags
  • 氨基酸1 to 314
  • 標簽His tag N-Terminus
  • • 生物活性

      Typical enzyme concentration to support conjugation?in vitro?is 100 nM-1 μM depending on conditions.

    • 應用

      SDS-PAGE

      Functional Studies

    • 純度>95% by SDS-PAGE .
      
      
    • 形式Liquid
    • • 功能E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
      • 組織特異性Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
      • 序列相似性Belongs to the ATG3 family.
      • 翻譯后修飾Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy.
      • 細胞定位Cytoplasm.