Size??100ug
Cat code??200493-2B5
Species Cross-reactivity??H,Mk
Key Application??WB,ICC
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG2a
Molecular Weight??46kDa
Sensitivity??This antibody detects endogenous levels of krt18 and does not cross-react with related proteins.
Immunogen??Purified recombinant human KRT18 protein fragments expressed in E.coli.
UniProt Number??P05783
GeneBank ID??NM_000224
Subcellular location??Cytoplasm ? perinuclear region
Formulation??Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol.
Altername??Cell proliferation-inducing gene 46 protein;Cytokeratin-18;Keratin-18;krt18;
Gene_symbol??K18;CYK18.
Summary??KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
UniPort summary  Function??Involved in the uptake of thrombin-antithrombin complexes by hepatic cells By similarity. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Application Images :??
Western blot detection of krt18 antibody in Hela,COS7 and 293T cell lysates using krt18 antibody (1:2000 diluted).Predicted band size:46KDa.Observed band size:46KDa.
Immunocytochemistry staining of HeLa cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-krt18 antibody (dilution 1:800).