Product: Anti-CAD (C-terminus), Polyclonal
Cat. No: PC-029 (100 μg)
Background:
Apoptosis is related to many diseases and
induced by a family of cell death receptors and
their ligands. Cell death signals are transduced
by death domain containing adapter molecules
and members of the caspase family of proteases.
These death signals finally cause the
degradation of chromosomal DNA by activated
DNase. A human DNA fragmentation factor
(DFF) was identified recently which was cleaved
by caspase-3 during apoptosis. Mouse homologue
of human DFF was identified as a DNase
inhibitor designated ICAD, for inhibitor of
caspase-activated Dnase. Upon cleavage of
DFF/ICAD, a caspase activated deoxyribonuclease
(CAD) is released and activated and
eventually causes the degradation of DNA in the
nuclei. Therefore, the cleavage of CAD inhibitor
molecule DFF/ICAD, which causes DNase
activation and DNA degradation, is the hallmark
of apoptotic cell death.
Specificity:
This antibody recognizes CAD.
Positive control: Murine lung or spleen tissue
lysate. A 40kDa band should be detected.
Species Reactivity:
Mouse, other species not tested.
Ig Isotype: Rabbit IgG
Immunogen:
A peptide corresponding to amino acids 314 to
329 of murine ICAD.
Format:
Affinity chromatography purified IgG, 100 μg in
200 μl of PBS containing 0.02% sodium azide.
Storage and Stability:
Stable for at least one year when stored at 4°C.
Applications and Suggested Dilutions:

Western blot: Suggested dilution: 1:500 to
1:1000.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.