His標簽抗體-抗體-抗體-生物在線
南寧市藍光生物技術有限公司
His標簽抗體

His標簽抗體

商家詢價

產品名稱: His標簽抗體

英文名稱: His Tag Antibody

產品編號: ICP0850

產品價格: null

產品產地: 加拿大

品牌商標: ImmuneChem

更新時間: null

使用范圍: ELISA WB IP IHC

南寧市藍光生物技術有限公司
  • 聯系人 :
  • 地址 : 廣西南寧市高新區科園大道68號東盟慧谷11棟203號
  • 郵編 : 530007
  • 所在區域 : 廣西
  • 電話 : 137****6451 點擊查看
  • 傳真 : 點擊查看
  • 郵箱 : 735565094@qq.com;info@bl-biotech.com

關于 His-Tag, Antibody 的詳細介紹

Catalog #

Product Description

ICP0850

This high affinity His-tag antibody is developed using a polyhistidine peptide (HHHHHH) conjugated to KLH and affinity-purified with the HHHHHH peptide on agarose. The antibody is useful for detection, affinity isolation, enrichment ?and localization of His-tag proteins.

Western-blot with His-tag ?antibody, HRP (ICP0851) of the His-tag protein (50 kDa) expressed cell lysate and enriched His-tag protein by IP with anti-His-tag (ICP0850) ? ? 1: Molecular marker ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 2: 10 μL of His-tag protein in lysate (original cell lysate) ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 3: 10 μL of His-tag protein in lysate (after IP) ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 4: 10 μL of the His-tag protein in 100 μL of the IP eluting buffer, enriched with 25 μg of the anti-His tag (ICP0850) from 2 mL of the original lysate
Species
Rabbit
Formulation
250 μg/mL in PBS, 50% glycerol
Immunogen
Polyhistidine peptide (HHHHHH) KLH conjugates
Purification
Antigen-specific affinity chromatography
Specificity
The antibody recognizes His-tag proteins.
Applications
ELISA (0.25-1 μg/mL); IP ( 1-10 μg, based on the sample size) ; WB (0.25-1.0 μg/mL); IHC (5 μg/mL)
Scientific Description
Polyhistidine peptide is frequently used as an affinity tag to facilitate the isolation of the 6H-fusion proteins using a metal-chelate matrix.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is three years from date of shipping if stored properly.

本公司還提供以下相關優質產品:

產品名稱(中文)* 產品名稱(英文) 品牌* 貨號* 規格*
β肌動蛋白抗體 Beta-Actin Antibody Immunechem CN0850 100ug
β微管蛋白抗體 Beta-Tubulin Antibody,HRP? Immunechem CN0865 100ug
GAPDH抗體 GAPDH Antibody? Immunechem CN0875? 100ug
谷胱甘肽抗體 Glutathione Antibody? Immunechem CN1005? 100ug
GST標簽抗體 GST Antibody? Immunechem CN0885? 100ug
Flg標簽抗體 Flg (DYKDDDDK) Antibody Immunechem ICP0901 100ug
FITC標記Flg標簽抗體 Flg (DYKDDDDK) Antibody, FITC? Immunechem ICP0903? 100ug
HRP標記Flg標簽抗體 Flg (DYKDDDDK) Antibody, HRP? Immunechem ICP0902? 100ug
HA標簽抗體 HA Antibody? Immunechem ICP0750? 100ug
HRP標記HA標簽抗體 HA Antibody, HRP? Immunechem ICP0751 100ug
His標簽抗體 His-Tag, Antibody? Immunechem ICP0850? 100ug
HRP標記His標簽抗體 His-Tag Antibody HRP? Immunechem ICP0851 100ug
偶聯Flg抗體的瓊脂糖填料 Flg (DYKDDDDK) Antibody,Agarose Immunechem ICP1315 1ml/支,10mg抗體
偶聯HA抗體的瓊脂糖填料 HA Antibody,Agarose Immunechem ICP1305 1ml/支,10mg抗體
偶聯His抗的瓊脂糖體填料 His-Tag Antibody,Agarose Immunechem ICP1310 1ml/支,10mg抗體