Anti-ERBB2 (D769H) Mouse Monoclonal Antibody-抗體-抗體-生物在線

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武漢紐斯特生物技術(shù)有限公司
Anti-ERBB2 (D769H) Mouse Monoclonal Antibody

Anti-ERBB2 (D769H) Mouse Monoclonal Antibody

商家詢價(jià)

產(chǎn)品名稱: Anti-ERBB2 (D769H) Mouse Monoclonal Antibody

英文名稱: Anti-ERBB2 (D769H) Mouse Monoclonal Antibody

產(chǎn)品編號(hào): 26381

產(chǎn)品價(jià)格: null

產(chǎn)品產(chǎn)地: 美國(guó)

品牌商標(biāo): NewEastBio

更新時(shí)間: null

使用范圍: ELISA、WB、 IHC

武漢紐斯特生物技術(shù)有限公司
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ERBB2 (D769H) [26381]
[100ul]
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Catalog Number:??26381
Gene Symbol:??ERBB2/HER2/Neu/c-Neu
Description: ??Anti-ERBB2 (D769H) Mouse Monoclonal Antibody
Background: ??The HER-2/neu oncogene, a member of the epidermal growth factor receptor or erb-B gene-like family, encodes a transmembrane tyrosine kinase receptor that mediates extracellular signals activated by epidermal growth factors. Her2 abnormal has been strongly associated with many malignant tumors, especially with breast cancers. The expression level of Her2 is an important critiria in clinic evaluating of the progression of breast cancer.
Immunogen: ??A synthetic peptide from the internal region of ERBB2 which includes the mutation of D769H, human origin.
Applications: ??ELISA, WB, IHC
Recommended Dilutions: ??
ELISA: 1:1000-1:2000?
WB: 1:500-1:1000?
IF: 1:25-1:50
Concentration: ??1 mg/ml
Host: ??Mouse
Format: ??Liquid
Clonality: ??Monoclonal
Isotype: ??
Purity: ??Purified from ascites
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??Recognizes D769H mutant, but not wild-type ERBB2 of vertebrates.
Storage Conditions:???Store at -20°C. Avoid freeze / thaw cycles
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Western blot:??
Western blot analysis of recombinant ERBB2 (D769H) and wildtype proteins. Purified His-tagged ERBB2 (D769H) protein (lane 1) and corresponding wildtype protein (lane 2) were blotted with anti-ERBB2 (D769H) monoclonal antibody (Cat. #26381).
Immunofluorescence:??
Immunofluorescence of cells expressing ERBB2 proteins with anti-ERBB2 (D769H) antibody. HEK293T cells were transfected with pCDNA3-GFP-ERBB2 (WT) plasmid (left column) or pCDNA3-GFP-ERBB2 (D769H) plasmid (right column), then fixed and stained with anti-ERBB2 (D769H) monoclonal antibody (Cat. #26381).