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該細胞系是1972年由GiardDJ通過肺癌組織移植培養建系的，源自一位58歲的白人男性。A549能通過胞苷二磷脂酰膽堿途徑合成富含不飽和脂肪酸的卵磷脂；角蛋白陽性。

細胞名稱：人非小細胞肺癌細胞

細胞簡稱：A549

產品貨號：TCH-C116

種屬來源：人

組織來源：肺

疾病特征：肺癌

細胞形態：上皮細胞樣

生長特性：貼壁生長

培養體系：D/F12+10%FBS+1%P/S

傳代比例：1:3-1:6，每2-3天換液一次

傳代周期：48-72 h

培養條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎培養基+30%FBS+10%DMSO，液氮儲存

質量檢測：細菌、真菌、支原體檢測均為陰性

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