In addition, Essential 8 Medium has been used to scale up production of iPSCs and shown to support iPSC growth for >50 passages without any signs of karyotypic abnormalities, along with maintaining the ability of iPSCs to differentiate into all three germ line lineages.

Features of Essential 8 Medium include:

 Consistentreduced variability compared to existing feeder-free culture media
 Robustreliable and robust cultures with a xeno-free, cGMP, 8-component medium
 Cost effectiveeconomical and scalable PSC culture compared to other feeder-free media

Reduced Variability:
Essential 8 Medium is xeno-free and contains only the 8 essential components needed for stem cell culture. Unlike other media that contain over 20 highly variable ingredients, Essential 8 Medium is produced under cGMP and has an optimized formulation and growth factor levels to help ensure maximum cell health, pluripotency, and growth, with minimal variability.

Reliable and Robust Cultures:
Essential 8 Medium has been show to support pluripotent stem cell growth and provide cultures with superior morphology and growth kinetics compared to other feeder systems.

Cost Effective:
Essential 8 Medium is provided in a convenient two-component kit (500 ml basal medium & 10 ml supplement), and when used with vitronectin (VTN-N), provides a cost effective, defined system for feeder-free culture of human pluripotent stem cells (PSCs).