Anti-NeuN抗體[EPR12763] - Neuronal Marker-抗體-抗體-生物在線
深圳市宇德立生物科技有限公司
Anti-NeuN抗體[EPR12763] - Neuronal Marker

Anti-NeuN抗體[EPR12763] - Neuronal Marker

商家詢價

產品名稱： Anti-NeuN抗體[EPR12763] - Neuronal Marker

英文名稱： FLJ56884 antibody FLJ58356 antibody Fox-1 homolog C antibody fox1 homolog C antibody Fox3 antibody FOX3NeuN antibody hexaribonucleotide binding protei

產品編號： ab177487

產品價格： null

產品產地： 英國

品牌商標： abcam

更新時間： 2023-09-21T08:08:01

使用范圍：

深圳市宇德立生物科技有限公司
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  • 經測試應用IHC-FoFr,?Flow Cyt,?IHC-Fr,?IHC-P,?WB,?ICC/IFmore details
  • 種屬反應性
    與反應:?Mouse, Rat, Sheep, Goat, Cat, Dog, Human, Zebrafish, Marmoset (common)
  • 免疫原

    Synthetic peptide within Human NeuN aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link:?A6NFN3

  • 陽性對照
    • This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Mouse Cerebellum; Rat cerebellum; Human Fetal brain as well as the following whole cell lysates: 293T, HeLa and SH-SY5Y lysates; SH-SY-5Y cells. Flow Cyt: U-87MG cells.
  • 常規說明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb??technology. RabMAb??technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-NeuN antibody (Alexa Fluor??488)?[EPR12763] - Neuronal Marker (ab190195)

    Anti-NeuN antibody (Alexa Fluor??647)?[EPR12763] - Neuronal Marker (ab190565)

性能

應用

Our?Abpromise guarantee?covers the use of?ab177487?in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

應用 Abreviews 說明
IHC-FoFr ? 1/500 - 1/6000.
Flow Cyt ? 1/100.
IHC-Fr ? 1/500.
IHC-P ? 1/3000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See protocols (link:?http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified use at 1/800.
WB ? 1/1000 - 1/10000. Detects a band of approximately 48,50 kDa (predicted molecular weight: 34 kDa).

For unpurified use at 1/1000 - 1/2000.
ICC/IF ? 1/300.

For unpurified use at 1/80.

靶標

  • 功能RNA-binding protein that regulates alternative splicing events.
  • 序列相似性Contains 1 RRM (RNA recognition motif) domain.
  • 細胞定位Nucleus. Cytoplasm.

  • Anti-NeuN antibody [EPR12763] - Neuronal Marker 圖像

    • ?An independent comparison of commercially available NeuN clones in IHC-Fr (acetone-fixed mouse dentate gyrus sections)

      ?

      Competitor A: Leading mouse monoclonal

      Competitor B: Non-Abcam rabbit monoclonal

      ?

      ?

      ab177487 produces intense, specific staining with minimal background, even at half the dilution of competing antibodies.