SUPEROSE 12
產品名稱： SUPEROSE 12
英文名稱： SUPEROSE 12
產品編號： 17-5173-01
產品價格： 0
產品產地： 美國
品牌商標： GE
更新時間： null
使用范圍： null
| 17-5172-01 | SUPEROSE 6 10/300 GL |
| 17-5173-01 | SUPEROSE 12 10/300 GL |
| 17-5174-01 | SUPERDEX 75 10/300 GL |
| 17-5175-01 | SUPERDEX 200 10/300 GL |
| 17-5176-01 | SUPERDEX PEPTIDE 10/300 GL |
| 28-9065-61 | Superdex 200 5_150 GL |
| 28-9205-04 | Superdex 75 5_150 GL |
| 17-0506-01 | PP COLUMN MONO Q HR 16/10 |
| 17-0507-01 | PP COLUMN MONO S HR 16/10 |
| 17-1002-01 | MONO Q 60/100 |
Superose? Columns and Lab Packs
- Achieve high-resolution separations across exceptionally broad molecular-weight ranges.
- Easy, efficient scale-up from prepacked Superose? 10/300 GL columns to Superose? prep grade lab packs.
- Low ionic strength eluents (< 0.05 M) can utilize a hydrophobic interaction component to alter the selectivity of Superose? for some lipids, peptides, and small aromatic compounds.
- Tricorn? high-performance column (10/300 GL) prepacked with Superose? allows even distribution of eluent across the column bed to enable high-resolution separations.
- Precision columns (PC) for micropurification and analysis?10-fold smaller column volume than Tricorn? columns
Superose? Columns and Lab Packs
Technical Information
Superose? 6 is designed for high-resolution chromatography. Superose? 6 prep grade permits easy, efficient scale-up to preparative separations. Superose? 12 is for high resolution purification of proteins from 1 × 103 to 3 × 105 molecular weight, while Superose? 12 prep grade is useful for preparative purification of these higher molecular weight proteins.
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| TECHNICAL SPECIFICATIONS | |
| Superose? 10/300 GL Columns (Tricorn?)* | |
| Bed volume? | 24 ml? |
| Sample loading capacity? | 5-10 mg, 240 μl? |
| Recommended flow rate? | ? |
| Superose? 6? | 0.3-0.5 ml/min? |
| Superose? 12? | 0.5-1.0 ml/min? |
| Max. pressure? | ? |
| Superose? 6? | 15 bar (217 psi, 1.5 MPa)? |
| Superose? 12? | 30 bar (435 psi, 3 MPa)? |
| Particle size? | ? |
| Superose? 6? | 13 ± 2 μm? |
| Superose? 12? | 10 ± 2 μm? |
| Storage? | 20% ethanol? |
| Storage temperature? | 4°C to 30°C? |
| Chemical stability? | Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)? |
| pH stability? | 3-12 (working and long term)? |
| ? | 1-14 (short term)? |
| Theoretical plates? | ? |
| Superose? 6? | > 30?000 m-1? |
| Superose? 12? | > 40?000 m-1? |
| Superose PC 3.2/30 | |
| Bed volume? | 2.4 ml ? |
| Max. loading capacity? | 200 μl? |
| Max. flow rate? | ? |
| Superose 6? | 0.1 ml/min? |
| Superose 12? | 0.1 ml/min? |
| Max. pressure? | ? |
| Superose 6? | 12 Bar (1.2MPa / 175 psi)? |
| Superose 12? | 24 Bar (2.4MPa / 350 psi)? |
| Particle size? | ? |
| Superose 6? | 13 ± 2 μm? |
| Superose 12? | 10 ± 2 μm? |
| Chemical stability? | Stable in all common buffers: 1?M acetic acid, 8?M urea, 6?M guanidine hydrochloride, 1% SDS, organic solvents, 0.5?M NaOH (for cleaning-in-place)? |
| pH stability? | 3-12 (working and long term)?1-14 (short term)? |
| Theoretical plates? | ? |
| Superose 6? | > 30 000 m-1? |
| Superose 12? | > 40 000 m-1? |
| * Columns are not suitable for use with ?KTAprime? plus system.
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| TECHNICAL SPECIFICATIONS | |
| Superose? prep grade | |
| Exclusion limit (Mr)? | ? |
| Superose? 6 prep grade? | 4 × 107 protein? |
| Superose? 12 prep grade? | 2 × 106 protein? |
| Separation range (Mr)? | ? |
| Superose? 6 prep grade? | 5 × 103-5 × 106 protein? |
| Superose? 12 prep grade? | 1 × 103-3 × 105 protein? |
| Matrix? | Highly cross-linked agarose? |
| Particle size? | 30 ± 10 μm? |
| Recommended linear flow rate? | ? |
| Superose? 6 prep grade? | up to 40 cm/h? |
| Superose? 12 prep grade? | up to 40 cm/h? |
| Max. pressure? | ? |
| Superose? 6 prep grade:? | 4 bar (58 psi, 0.4 MPa)? |
| ? Superose? 12 prep grade:? | 7 bar (101 psi, 0.7 MPa)? |
| Storage? | 20% ethanol? |
| Storage temperature? | 4°C to 30°C? |
| * Columns are not suitable for use with ?KTAprime? plus system.
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. Numbers above peaks correspond to the number of base pairs. Reproduced by kind permission of the authors and publisher.'))
Separation of DNA fragments on Superose? 6 HR 10/30 column (now available as Superose 6 10/300 GL column). Numbers above peaks correspond to the number of base pairs. Reproduced by kind permission of the authors and publisher.
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References
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- Andersson, T et al. Agarose-based media for high-resolution gel filtration of ?biopolymers. J. Chromatogr. 326, 33 (1985).
- Ellegren, H. and L??s, T. Size-exclusion chromatography of DNA restriction fragments. ?J. Chromatogr. 467, 217 (1989).
- Gao, Y. et al. A cytoplasmic chaperonin that catalyzes beta-actin folding. Cell 69, 1043 (1992).
- Lin, M. et al. Pyruvate kinase isoenzymes from Green Alga, Selenastrum minutum. Arch. Biochem. Biophys. 269, 219 (1989).
- Hei, Y. et al. Purification and characterisation of a novel ribosomal S6 kinase from skeletal muscle of insulin-treated rats. J. Biol. Chem. 269, 7816 (1994).