• 特異性No cross reactivity with other proteins.
• 經測試應用IHC-P,?WB,?ICCmore details
• 種屬反應性
  與反應:?Human
  預測可用于:?Mouse, Rat, Sheep, Cow
• 免疫原

  Synthetic peptide corresponding to Human 14-3-3 sigma aa 140-156 (internal sequence).
  Sequence:

  KKRIIDSARSAYQEAMD

  
  Database link:?P31947

  ?

  Run BLAST with?Run BLAST with?
• 陽性對照
  • Human tonsil and oesophagus squama cancer tissue. HeLa, A549, COLO320 amd SE620 cell lysates.

性能

• 形式Liquid
• 存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存儲溶液Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
  Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
• 濃度100 μg 濃度為 0.5 mg/ml?
  
• 純度Immunogen affinity purified
• 克隆多克隆
• 同種型IgG
• 研究領域
  • Neuroscience
  ?
  • Neurology process
  ?
  • Neural Signal Transduction

  • Signal Transduction
  ?
  • Adapters
  ?
  • Cytoplasmic

  • Neuroscience
  ?
  • Cell Type Marker
  ?
  • Neuron marker
  ?
  • Synapse marker

  • Stem Cells
  ?
  • Signaling Pathways
  ?
  • Wnt
  ?
  • 14-3-3

應用

靶標

• 功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
  p53-regulated inhibitor of G2/M progression.
• 組織特異性Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
• 序列相似性Belongs to the 14-3-3 family.
• 翻譯后修飾Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
• 細胞定位Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
• Information by UniProt
• 數據庫鏈接
  • Entrez Gene: 528453?Cow
  • Entrez Gene: 2810?Human
  • Entrez Gene: 55948?Mouse
  • Entrez Gene: 313017?Rat
  • Entrez Gene: 443032?Sheep
  • Omim: 601290?Human
  • SwissProt: Q0VC36?Cow
  • SwissProt: P31947?Human

  see all
• 別名
  • 14 3 3 protein sigma antibody
  • 14-3-3 protein sigma antibody
  • 1433S_HUMAN antibody

  see all

Anti-14-3-3 sigma antibody 圖像

• 

  Western blot - Anti-14-3-3 sigma antibody (ab190960)
  All lanes :?Anti-14-3-3 sigma antibody (ab190960) at 0.5 μg/ml
  
  Lane 1 :?HeLa cell lysate
  Lane 2 :?A549 cell lysate
  Lane 3 :?A549 cell lysate
  Lane 4 :?COLO320 cell lysate
  Lane 5 :?SE620 cell lysate
  
  
  Predicted band size :?27 kDa
  
• 

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