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Human Growth Factors & Cytokines
Recombinant Human sCD40 Ligand





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		Synonyms :
soluble CD40 Ligand, TNFSF5, TRAP, CD154, gp39, T-BAM



		Description :
CD40, a member of the TNF receptor family, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells.  Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching.  The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes.  Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE.  The human CD40L gene codes for a 261 amino acid type II transmembrane protein, which contains a 22 amino acid cytoplasmic domain, a 24 amino acid transmembrane domain, and a 215 amino acid extracellular domain.  The soluble form of CD40L is an 18 kDa protein comprising the entire TNF homologous region of CD40L and is generated in vivo by an intracellular proteolytic processing of the full length CD40L.  Recombinant human soluble CD40 ligand is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.



		Catalog #:
310-02



		Source :
E.coli



		Purity :
Greater than 98% by SDS-PAGE gel and HPLC analyses.



		Endotoxin Level :
Endotoxin level is less than 0.1 ng per μg (1EU/μg).



		Biological Activity :
Assay #1:  Determined by the stimulation of IL-12 induction by human PBMCs. 
 Assay #2:  Determined by the dose-dependent stimulation of IL-8 production by human PBMC.  The expected ED50 for this effect is 5-10 ng/ml.  Note:  Results may vary with PBMC donors.


Selected References :
Selected List of References Citing This PeproTech Product



                AA Sequence :
MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL 



		PubMed Link :
CD40+AND+ligand


Country of Origin :
USA
















        Size A
10μg
$80?
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        Size B
50μg
$195?
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        Size C
1mg
$1500?
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Special 
                    Sizes: 
 Many products can be ordered in custom sizes. Please click here to contact Peprotech with your request.





Usage:
For Research Use Only.  Not for use in diagnostic or therapeutic procedures.