DYKDDDDK tag monoclonal antibody, clone 29E4.G7.H11
產品名稱： DYKDDDDK tag monoclonal antibody, clone 29E4.G7.H11
英文名稱： DYKDDDDK tag monoclonal antibody, clone 29E4.G7.H11
產品編號： MAB2094
產品價格： null
產品產地： 臺灣
品牌商標： Abnova
更新時間： null
使用范圍：
亞諾法生技股份有限公司（Abnova)
- 聯系人 :
- 地址 : 臺灣臺北市內湖區洲子街 108 號 9 樓
- 郵編 : 11493
- 所在區域 : 臺灣
- 電話 : +886-920**1152 點擊查看
- 傳真 : 點擊查看
- 郵箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against synthetic peptides of FLAG epitope tag.
- Immunogen:
- A synthetic peptide corresponding to FLAG epitope tag.
- Host:
- Mouse
- Form:
- Liquid
- Isotype:
- IgG
- Storage Buffer:
- In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:160000)
Western Blot (1:2000-1:20000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- A strategy for the expression of recombinant proteins traditionally hard to purify.
Frank EG, McDonald JP, Karata K, Huston D, Woodgate R.Anal Biochem. 2012 Jul 22.
- 2.
- Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG monoclonal antibodies M1, M2 and M5.
Slootstra JW, Kuperus D, Pluckthun A, Meloen RH.Mol Divers. 1997;2(3):156-64.
- 3.
- Double tagging recombinant A1- and A2A-adenosine receptors with hexahistidine and the FLAG epitope. Development of an efficient generic protein purification procedure.
Robeva AS, Woodard R, Luthin DR, Taylor HE, Linden J.Biochem Pharmacol. 1996 Feb 23;51(4):545-55.
- 4.
- Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells.
Chubet RG, Brizzard BL.Biotechniques. 1996 Jan;20(1):136-41.
- Applications
- Western Blot (Recombinant protein)
- DYKDDDDK tag monoclonal antibody, clone 29E4.G7.H11 (Cat # MAB2094) to detect FLAG? conjugated proteins detects both C terminal linked and N terminal linked FLAG? tagged recombinant proteins by western blot.
