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產品基本信息

產品編碼：A10938R

英文名稱：beta-Actin (Loading Control)

中文名稱：β-肌動蛋白/β-Actin 抗體（內參抗體）

別名：Beta Actin; beta-Actin; ACTB; Actin cytoplasmic 1; Actin, beta; Beta actin; beta cytoskeletal actin;A X actin like protein; ACTB; Actin cytoplasmic 1; alpha sarcomeric Actin; Actx; Beta cytoskeletal actin; Melanoma X actin; PS1TP5BP1.

市場價格：0.1ml/480元 ? ? ?0.2ml/800元

產品類型內參抗體?

研究領域腫瘤 ?細胞生物 ?免疫學 ?信號轉導 ?細胞骨架 ?

抗體來源Rabbit

克隆類型Polyclonal

交叉反應Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep, Fish, Guinea Pig, Hamster, Cat, mt,op

產品應用WB=1:1000-1500 ELISA=1:500-1000 IP=1:20-200 IHC-P=1:100-1000 IHC-F=1:100-1000 IF=1:100-1000 （石蠟切片需做抗原修復）?

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量42kDa

細胞定位細胞漿?

性 ? ?狀Lyophilized or Liquid

濃 ? ?度1mg/1ml

免 疫 原Synthetic MAP peptide derived from human beta-Actin

亞 ? ?型IgG

純化方法affinity purified by Protein A

儲 存 液0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMedPubMed

產品介紹background:

Loading Control

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008].

Function:

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Subunit:

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2.

Subcellular Location:

Cytoplasm. cytoskeleton.

Tissue Specificity:

Ubiquitously expressed in all eukaryotic cells.

Post-translational modifications:

ISGylated.

Oxidation of Met-44 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.

DISEASE:

Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.

Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.

Similarity:

Belongs to the actin family.