Product: Anti-Caspase-8, Clone FLICE-3
Cat. No: MC-465 (100 μg)
Background:
Caspases are key effectors of programmed cell
death. They are synthesized as inactive proenzymes
which are activated by cleavage at a
specific aspartate residue to form two subunits.
These subunits are normally linked together by a
linker, which may be involved in the regulation of
the different caspases. Caspase-8 is a key
enzyme in the apoptosis pathway. Caspase-8
contains two N-terminal region death effector
domains, which are removed to activate the
enzyme along with cleavage in the two subunits.
These subunits then form the active protease,
which is capable of cleaving caspase-3, -6 and -
7. These caspases are then capable of cleaving
other cellular substrates such as PARP and DFF,
which in turn induce apoptosis.
Ig Isotype: IgG1
Immunogen:
Synthetic peptide corresponding to amino acids
1 to 9 of human caspase-8.
Format:
Mouse monoclonal antibody against human
Caspase-8 (cysteine-requiring aspartate
protease-8). Available in 100 μl vials at a
concentration of 1 mg/ml (100 μg) in PBS with
0.08% sodium azide. Purified by 0.2 μm sterile
filtration.
Storage and Stability:
Stable for 24 months when stored at -20°C.
Aliquot to avoid repeat freeze/thaw cycles.
Applications and Suggested Dilutions:

Western Blot
The optimal dilution for a specific application
should be determined by the researcher.
References:
1. Cohen G.M., et al. (1997) Caspases: the
executioners of apoptosis. Biochem. J. 326:
1-16.
2. Martin D.A., et al. (1998) Membrane
oligomerization and cleavage activates the
caspase-8 (FLICE/MACHalpha1) death
signal. J. Biol. Chem. 273: 4345-4349.
3. Green D.R., et al. (1998) Apoptotic
pathways: the roads to ruin. Cell 94: 695-
698.
4. Hu S., et al. (1997) I-FLICE, a novel
inhibitor of tumor necrosis factor receptor-1-
and CD-95-induced apoptosis. J. Biol.
Chem. 272: 17255-17257.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product