Tubulin-alpha monoclonal antibody, clone TU-01
產品名稱： Tubulin-alpha monoclonal antibody, clone TU-01
英文名稱： Tubulin-alpha monoclonal antibody, clone TU-01
產品編號： MAB3826
產品價格： null
產品產地： 臺灣
品牌商標： Abnova
更新時間： null
使用范圍：
亞諾法生技股份有限公司（Abnova)
- 聯系人 :
- 地址 : 臺灣臺北市內湖區洲子街 108 號 9 樓
- 郵編 : 11493
- 所在區域 : 臺灣
- 電話 : +886-920**1152 點擊查看
- 傳真 : 點擊查看
- 郵箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against native Tubulin-alpha.
- Immunogen:
- Native purified Tubulin-alpha from porcine brain alpha tubulin (two cycles of polymerization-depolymerization).
- Host:
- Mouse
- Reactivity:
- Pig
- Specificity:
- This antibody recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.
- Form:
- Liquid
- Concentration:
- 1 mg/mL
- Isotype:
- IgG1
- Storage Buffer:
- In PBS, pH 7.4 (15 mM sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- Western Blot (1-2 mg/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5 mg/mL)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Dazap2 modulates transcription driven by the Wnt effector TCF-4.
Lukas J, Mazna P, Valenta T, Doubravska L, Pospichalova V, Vojtechova M, Fafilek B, Ivanek R, Plachy J, Novak J, Korinek V.Nucleic Acids Res. 2009 May;37(9):3007-20. Epub 2009 Mar 20.
- 2.
- Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells.
Kukharskyy V, Sulimenko V, Macurek L, Sulimenko T, Draberova E, Draber P.Exp Cell Res. 2004 Aug 1;298(1):218-28.
- 3.
- Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells.
Smertenko A, Blume Y, Viklicky V, Opatrny Z, Draber P.Planta. 1997;201(3):349-58.
- Applications
- Western Blot (Cell lysate)
- Immunoprecipitation of alpha-tubulin from HeLa and RAJI cell lysate by antibody TU-16 and its detection by antibody TU-01 (Cat # MAB3826).
IgM heavy chain and IgM light chain (25-30 KDa) indicated.
MW of alpha tubulin is around 50 KDa.
L = lysate. IPr = immunoprecipitate (reducing conditions). IPn = immunoprecipitate (non-reducing conditions).
- Western Blot (Cell lysate)
- Use of alpha Tubulin monoclonal antibody, clone TU-01 (Cat # MAB3826) as a loading control (A) in an Western blotting experiment revealing the staining pattern of various cell lysates by a newly developed monoclonal antibody (B).
- Immunofluorescence
- Immunofluorescence staining of HeLa (human cervix carcinoma cell line) using alpha Tubulin monoclonal antibody, clone TU-01 (Cat # MAB3826 ; red). Nucleus is stained with DAPI (blue).
- Immunofluorescence
- Panel A : Immunofluorescence staining of NIH/3T3 (mouse embryonal fibroblast cell line) using alpha Tubulin monoclonal antibody, clone TU-01 (Cat # MAB3826 ; green) and VIM monoclonal antibody, clone VI-01 (Cat # MAB3880 ; red). Nucleus is stained with DAPI (blue).
Panel B : Immunofluorescence staining of NIH/3T3 (mouse embryonal fibroblast cell line) using alpha Tubulin monoclonal antibody, clone TU-01 (Cat # MAB3826 ; green). Nucleus is stained with DAPI (blue).