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本蛋白可以用于診斷試劑開發和受體研究

純度

> 90 % as determined by SDS-PAGE.

內毒素

< 1.0 EU per μg protein as determined by the LAL method.

生物活性

The 2019-nCoV Spike Protein (S1 Subunit, His Tag) can bind with Human ACE2 in ELISA assay.

蛋白構建

A DNA sequence encoding the NCP-CoV(2019-nCoV) spike protein S1 Subunit was expressed with a polyhistidine tag at the C-terminus.

表達宿主

HEK293 Cells

種屬

2019-nCoV

預測 N 端

Val

蛋白質氨基酸序列：

VNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYEHHHHHH

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分子量：

The recombinant NCP-CoV(2019-nCoV) Spike Protein (S1 Subunit, His Tag) consists of 652 amino acids and predicts a molecular mass of 73.25 kDa.

緩沖液

Lyophilized from sterile PBS, pH 7.4.

運輸方式

proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

穩定性 & 儲存條件

Samples are stable for up to twelve months from date of receipt at -20℃?to -80℃
Store it under sterile conditions at -20℃?to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.