PODS® Human GDNF 膠質細胞源性神經營養因子
產品名稱： PODS® Human GDNF 膠質細胞源性神經營養因子
英文名稱： PODS® Human GDNF (Glial cell derived neurotrophic factor)
產品編號： PPH2
產品價格： 0
產品產地： 英國
品牌商標： Cell Guidance Systems
更新時間： null
使用范圍： null
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PODS? Human GDNF
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| PODS? co-crystals? | PODS? co-crystals? |
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CellGS Catalogue Code? ? ? ? |
Product Name?? ? ? ? ? ? ? ? |
Pack Size? ? ? ? ? ? ? |
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PPH2-50?? ? ? ? ? ? ? ? |
PODS?Human GDNF??? ? ? ? ? ? ? ? |
50 million? ? ? ? ? ?? |
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PPH2-250?? ? ? ? ? ? ? ? |
PODS?Human GDNF??? ? ? ? ? ? ? ? |
250 million? ? ? ? ?? |
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PPH2-1000?? ? ? ? ? ? ? ? |
PODS?Human GDNF??? ? ? ? ? ? ? ? |
1 billion? ? ? ? ? ? |
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PODS 技術
將生長因子包埋進蛋白質晶體（多角體蛋白）中，多角體蛋白能夠保護包埋的生長因子，并維持其穩定性與生物活性，進而持續釋放到細胞培養系統中。
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PODS GDNF 描述
PODS多角體蛋白與GDNF共結晶。GDNF (Glial cell derived neurotrophic factor，膠質細胞源性神經營養因子)是由Lin等(1993)從鼠膠質細胞株B49的條件培養基中分離純化獲得的一種神經營養因子，且由此而命名。以純化的GDNF的氨基端序列制作探針，克隆得到大鼠和人的GDNF基因。人GDNF前體蛋白為211個氨基酸殘基(其中信號肽19個氨基酸)，加工處理后才形成分泌型的成熟蛋白，有134個氨基酸，是一個糖基化的二硫鍵連結的同源二聚體蛋白質，分子量為32~34kD，為堿性蛋白質。
The product contains the polyhedrin protein co-crystalized with Human GDNF. Glial cell-derived neurotrophic factor (GDNF) is a neurotrophic factor that signals through a multicomponent receptor system to activate receptor tyrosine kinase RET signaling. GDNF promotes dopamine uptake, prevents motor neuron apoptosis, and supports the survival and differentiation of neurons.
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PODS GDNF 規格
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PODS GDNF Product ? Details?? ? ? ? ? ? ? ? |
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Length?? ? ? ? ? ? ? ? |
180 aa?? ? ? ? ? ? ? ? |
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Structure?? ? ? ? ? ? ? ? |
Dimer?? ? ? ? ? ? ? ? |
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Source?? ? ? ? ? ? ? ? |
Spodoptera ? frugiperda (Sf9) cell culture?? ? ? ? ? ? ? ? |
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Accession ? Number? ? ? |
P39905? ? ? ? ? ? ? ?? |
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Endotoxin ? Level? ? ?? |
<0.06 EU/ml ? as measured by gel clot LAL assay?? ? ? ? ? ? ? ? |
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Formulation?? ? ? ? ? ? ? ? |
PODS? were ? lyophilized from a volatile solution?? ? ? ? ? ? ? ? |
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Reconstitution?? ? ? ? ? ? ? ? |
PODS? ? co-crystals may be reconstituted at 200 million co-crystals/ml in water. 20% ? glucose has a buoyant density closer to PODS? co-crystals and can be useful ? for aliquoting.?? ? ? ? ? ? ? ? PODS? ? co-crystals are highly stable when stored in aqueous solution (pH range 6 - ? 8).?? ? ? ? ? ? ? ? |
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Stability and ? Storage? |
Upon receipt, ? store at 4°C. PODS? co-crystals are stable for at least 1 year when dry and 6 ? months when resuspended.?? ? ? ? ? ? ? ? |
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PODS GDNF 使用建議
PODS? co-crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biological activity of 50 million PODS? co-crystals generates the same peak dose as 3.3 μg of standard recombinant protein. However, at 5 days following the start of seeding the PODS? co-crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS? co-crystals that is optimal for a particular experiment should be determined empirically. Based on previous data, we suggest using?50 million PODS? co-crystals in place of 3.3 μg of standard growth factor?as a starting point."
To control for cross-reactivity with cells or as a negative control, we recommend using PODS? growth factors alongside PODS? Empty crystals, as the latter do not contain or release cargo protein.
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PODS GDNF AA Sequence
MADVAGTSNR DFRGREQRLF NSEQYNYNNS KNSRPSTSLY KKAGLMSPDK QMAVLPRRER NRQAAAANPE NSRGKGRRGQ RGKNRGCVLT AIHLNVTDLG LGYETKEELI FRYCSGSCDA AETTYDKILK NLSRNRRLVS DKVGQACCRP IAFDDDLSFL DDNLVYHILR KHSAKRCGCI
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PODS GDNF Alternative Names
Glial cell derived neurotrophic factor, ATF, HFB1-GDNF, ATF1, ATF-1, astrocyte-derived trophic factor 2,? 膠質細胞源性神經營養因子
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PODS GDNF? 文件資料
 PPH2 Datasheet?? ? ? ? ? ? |
PODS User Guide?? ? ? ? ? ? |
PODS MSDS?? ? ? ? ? ? |
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更多PODS 相關產品：PODS 生長因子
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