Size??100ug
Cat code??200453
Species Cross-reactivity??H
Key Application??WB,ICC
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG1
Molecular Weight??37kDa
Sensitivity??This antibody detects endogenous levels PP1A (N-terminus) and does not cross-react with related proteins.
Immunogen??Purified recombinant human PP1A (N-terminus) protein fragments expressed in E.coli.
UniProt Number??P62136
GeneBank ID??NM_001008709
Subcellular location??Cytoplasm. Nucleus
Formulation??Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername??PP-1A; PPP1A; MGC1674; MGC15877; PP1alpha;Protein Phosphatase 1 alpha;PPP1CA
Gene_symbol??PP-1A; PPP1A; PP1alpha
Summary??The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniPort summary  Function??Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role. in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
Application Images :??
Western blot detection of PP1A(N-terminus) antibody in HepG2,MCF7&Hela cell lysates using PP1A(N-terminus) antibody (1:1000 diluted).
Predicted band size:37KDa.
Observed band size:37KDa.
Immunocytochemistry of HeLa cells using anti-PP1A (N-terminus) antibody diluted 1:50.