GST MULTITRAP FF
產品名稱： GST MULTITRAP FF
英文名稱： GST MULTITRAP FF
產品編號： 28-4055-01
產品價格： 0
產品產地： 美國
品牌商標： GE
更新時間： null
使用范圍： null
| 28-4055-00 | GST MULTITRAP 4B |
| 28-4055-01 | GST MULTITRAP FF |
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| 28-9523-60 | GST GraviTrap |
| 28-9523-61 | GST Buffer Kit |
| 28-9545-49 | PGEX-4T-1 |
| 28-9545-50 | PGEX-4T-2 |
| 28-9545-52 | PGEX-4T-3 |
| 28-9545-53 | PGEX-5X-1 |
| 28-9545-54 | PGEX-5X-2 |
| 28-9545-55 | PGEX-5X-3 |
| 28-9546-46 | PGEX-2TK |
| 28-9546-48 | PGEX-6P-1 VECTOR |
| 28-9546-50 | PGEX-6P-2 VECTOR |
| 28-9546-51 | PGEX-6P-3 VECTOR |
| 28-9546-53 | PGEX-2T 25 UG WI/DI |
| 28-9546-54 | PGEX-3X 25 UG WI/DI |
| 28-9546-56 | PGEX-1LT ECOR I/BAP |
| 11-0033-99 | HIS GRAVITRAP |
| 11-0034-00 | HIS BUFFER KIT |
| 27-4710-01 | ANTI-HIS ANTIBODY |
| 28-4009-89 | HIS MULTITRAP HP |
| 28-4009-90 | HIS MULTITRAP FF |
| 28-4013-53 | HIS SPINTRAP |
| 28-4039-43 | COLLECTION PLATE |
| 28-9321-71 | His SpinTrap Kit |
| 27-1583-01 | LIGHT PRIMER MIX |
| 27-1586-01 | HEAVY PRIMER MIX |
| 27-1588-01 | LINKER-PRIMER MIX |
| 27-1589-01 | RS PRIMER MIX |
| 27-9420-01 | ANTI-M13 MONOCLONAL ANTIBODY |
| 27-9421-01 | HRP/ANTI-M13 MONOCLNL CONJUG. |
GST MultiTrap FF
GST MultiTrap FF and GST MultiTrap 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and rapid parallel purification of glutathione S-transferase (GST) tagged proteins.
- 96-well filter plate format for high-throughput screening and parallel purification of GST-tagged* proteins.
- Highly reproducible well-to well and plate-to-plate results.
- High purity in one step, high chemical stability. Load unclarified samples directly to the wells.
- Wells are prepacked with Glutathione Sepharose media for convenience.
- Easy and predictable scale-up using prepacked GSTrap FF, GSTPrep FF 16/10, and GSTrap 4B columns.
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GST MultiTrap FF and GST MultiTrap 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and purification of glutathione S-transferase (GST) tagged proteins.
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Purification of up to 0.5 mg of GST-tagged proteins/well directly from unclarified cell lysate can be achieved using GST MultiTrap filter plates. Direct purification of GST-tagged proteins from unclarified lysate shortens handling time and minimizes degradation of sensitive target proteins. The 96-well plate format gives great flexibility, both when working with automated robotic systems and manually using centrifugation or vacuum. Consistent well-to-well and plate-to-plate performance ensures high reproducibility.
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GST MultiTrap FF is prepacked with Glutathione Sepharose 4 fast flow, while GST MultiTrap 4B is prepacked with Glutathione Sepharose 4B.
GST MultiTrap FF — Technical Specifications
| Complete Packsize | 4 x 96-well filter plates |
| Storage Conditions | 4 - 30°C, 20% Ethanol |
| Centrifugation Force Recommended | 100 x g-500 x g |
| Centrifugation Force Max. | 700 x g |
| ? | ? |
| Media | ? |
|---|---|
| Ligand | Glutathione and 10-carbon linker arm |
| Average Particle Size | 90 μm |
| Matrix | Highly cross-linked 4% agarose |
| Binding Capacity/ml Chromatography Medium | ≈ 10 mg recombinant GST/ml medium1) |
| pH stability Working Range | 3-12 |
| pH stability Cleaning | 3-12 |
| Pressure/Flow Specification | base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm |
| Flow Velocity | <450 cm/h |
| Ligand Coupling Method | Epoxy activation |
| Storage Conditions | 4 to 30°C, 20% Ethanol |
| Chemical Stability | All commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol or 6 M guanidine-hydrochloride for 1 h at room temperature |
| 1)Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution. | ? |
| ? | ? |
| Column | ? |
|---|---|
| Complete Packsize | 4 x 96-well filter plates |
| Number of Wells | 96 |
| Sample Volume | 600 μl/well |
| Reproducibility Between Wells | ± 10% in yield |
| Material | Polypropylene (PP) and polyethylene (PE). |
| Dimensions | 127.8 x 85.5 x 30.6 mm |