重組人PD-L1蛋白（Recombinant Human PD-L1 Protein），GMP級

產品貨號：GMP-TL727

產品描述（Description）

Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.

分子別名（Synonym）

CD274、B7-H1、PDCD1L1、PDCD1LG1

表達宿主（Expression host）

HEK293細胞

來源（Source）

Human

蛋白序列（Protein Sequence）

DNA序列編碼人PD-L1（NCBI Reference Sequence:NP_054862.1）表達帶有His標簽在C末端

分子量（Molecular Weight）

重組人PD-L1蛋白 包含222個氨基酸，預測理論分子量為26.9 kD

純度（Purity）

≥95%采用SDS-PAGE凝膠和高效液相色譜分析

內毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定 PD-L1(His 標簽)1ug/mL(100 uL/孔)能夠結合抗人PD-L1抗體的線性范圍是0.5-5 ng/mL

穩定性（Stability）

凍干制劑可在 4℃保存 24 個月， 溶解后的液體可置于-20℃保存 6-12 個月， 避免反復凍融

組成（Component）

含有6%甘露醇的無菌PBS（PH 7.4）的凍干粉

參考文獻

1. DNA double-strand break repair pathway regulates PD-L1 expression in cancer cells Hiro Sato, Atsuko Niimi, Takaaki Yasuhara, Tiara Bunga Mayang Permata, Yoshihiko Hagiwara, Mayu Isono, Endang Nuryadi, Ryota Sekine, Takahiro Oike, Sangeeta Kakoti, Yuya Yoshimoto, Kathryn D. Held, Yoshiyuki Suzuki, Koji Kono, Kiyoshi Miyagawa, Takashi Nakano, Atsushi Shibata Nat Commun. 2017; 8: 1751. Published online 2017 Nov 24. doi: 10.1038/s41467-017-01883-9

  注意：For research and manufacturing use.