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Size??100ug
Cat code??200135
Species Cross-reactivity??H,M
Key Application??WB,IP
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG1
Molecular Weight??63kDa
Sensitivity??This antibody detects endogenous levels of CARM1(C-terminus) and does not cross-react with related proteins.
Immunogen??Purified recombinant human CARM1(C-terminus) protein fragments expressed in E.coli.
UniProt Number??Q86X55
GeneBank ID??NM_199141
Subcellular location??Nucleus. Cytoplasm
Formulation??Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.2% sodium azide,0.1mg/mlBSA and 50% glycerol.
Altername??CARM1;Coactivator associated arginine methyltransferase 1;Histone arginine methyltransferase;Histone-arginine methyltransferase CARM1;PRMT4;Protein arginine N methyltransferase 4.
Gene_symbol??PRMT4
Summary??Protein arginine N-methyltransferases,such as CARM1,catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine.Protein arginine methylation has been implicated in signal transduction,metabolism of nascent pre-RNA,and transcriptional activation.
UniPort summary  Function??Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at ′Arg-17′ (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at ′Arg-2142′, which may loosen its interaction with NCOA2/GRIP1, and at ′Arg-580′ and ′Arg-604′ in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs
Application Images :??
Western blot detection of CARM1(C-terminus) antibody in Hela,A431 & K562 cell lysates using CARM1(C-terminus) antibody (1:200-1:500 diluted).Predicted band size:63KDa.Observed band size:63KDa.
Immunoprecipitation analysis of Hela cell lysates using CARM1 antibody.