MembraneProteinPurification Kit-色譜介質(zhì)-耗材-生物在線

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MembraneProteinPurification Kit

MembraneProteinPurification Kit

商家詢價

產(chǎn)品名稱： MembraneProteinPurification Kit

英文名稱： MembraneProteinPurification Kit

產(chǎn)品編號： 28-9805-82

產(chǎn)品價格： 0

產(chǎn)品產(chǎn)地： 美國

品牌商標： GE

更新時間： null

使用范圍： null

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  • 聯(lián)系人 :
  • 地址 : 上海市寶山區(qū)友誼路1506號東智廣場1109室
  • 郵編 : 201900
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  • 郵箱 : 342786402@qq.com

28-9805-82 MembraneProteinPurificationKit
17-5468-15 HiScreen Capto SP ImpRes
17-5470-15 HiScreen Capto Q ImpRes
17-5474-15 HiScreen MabSelect SuRe LX
28-9269-73 HiScreen MabSelect?
28-9269-76 HiScreen MabSelectXtra
28-9269-77 HiScreen MabSelectSuRe
28-9269-78 HiScreen Capto Q
28-9269-79 HiScreen Capto S
28-9269-80 HiScreen Capto MMC
28-9269-81 HiScreen Capto Adhere
28-9269-82 HiScreen Capto DEAE
28-9269-84 HiScreen Butyl FF
28-9269-85 HiScreen Butyl-S FF
28-9269-86 HiScreen Octyl FF
28-9269-88 HiScreen Phenyl FF, high sub
28-9269-89 HiScreen Phenyl FF, low sub
28-9505-10 HiScreen Q FF
28-9505-11 HiScreen Q HP
28-9505-13 HiScreen SP FF
28-9505-15 HiScreen SP HP
28-9505-16 HiScreen Phenyl HP
28-9505-17 HiScreen IMAC FF
28-9782-43 HiScreen Blue FF
28-9782-44 HiScreen Ni FF
28-9924-72 HiScreen Capto Phenyl (high su
28-9924-74 HiScreen Capto Blue

Membrane Protein Purification Kit

  • Simplify detergent screening of histidine-tagged membrane proteins.
  • Simultaneous evaluation of solubilization and purification efficiency.
  • Rapid and easy screening in the convenient magnetic bead format.
  • Ready-to-use detergent solutions.
  • Easy-to-follow protocol.

Technical Information


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Membrane Protein Purification Kit for histidine-tagged membrane proteins allows rapid and easy detergent screening for simultaneous evaluation of solubilization and purification efficiency.

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Membrane Protein Purification Kit is designed for efficient detergent screening of histidine-tagged membrane proteins. Small aliquots of cell membranes are solubilized in different detergents followed by rapid purification using His Mag Sepharose? Ni. The purification step is performed directly after solubilization using the same detergent. Analysis and evaluation can be performed by a number of methods such as Western blot, gel filtration, or light scattering. Membrane Protein Purification Kit is sufficient for two complete screening experiments according to the included protocol.

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The magnetic bead format is excellent for small-scale experiments. The high density of the beads allows rapid capture by magnetic devices, while the visibility of the beads ensures reliable collection of the bound histidine-tagged membrane proteins in the screening and purification procedure. Purification of target membrane proteins for a number of analytical and/or other downstream applications can be achieved within a few hours. For convenient purification of larger amounts of membrane proteins, use prepacked HisTrap? HP columns.

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TECHNICAL SPECIFICATIONS ? ? ?
Kit Contains* Amount supplied CMC?
(%)		 CMC?
(mM)		 Detergent
class
His Mag Sepharose Ni? 3 x 1 ml vial? ? ? ?
n-Dodecyl-b-D-maltoside (DDM) ? 10% (w/v) aqueous solution, 1 ml? 0.009? 0.17? Non-ionic?
n-Decyl-b-D-maltoside (DM)? 10% (w/v) aqueous solution, 1 ml? 0.09? 1.8? Non-ionic?
Lauryldimethylamine-N-oxide (LDAO)? 10% (w/v) aqueous solution, 1 ml? 0.02? 1-2? Zwitter-ionic?
n-Dodecylphosphocholine (FOS12) ? 10% (w/v) aqueous solution, 1 ml? 0.05? 1.5? Non-ionic?
Dodecyl octaethyleneglycol ether (C12E8) ? 10% (w/v) aqueous solution, 1 ml? 0.005? 0.09? Non-ionic?
Cyclohexyl-1-pentyl-b-D-maltoside (Cymal ?5)? 10% (w/v) aqueous solution, 1 ml? 0.12? 2-5? Zwitter-ionic?
n-Octyl-b-D-glucoside (OG)? 20% (w/v) aqueous solution, 1 ml? 0.53? 18-20? Non-ionic?
Phosphate buffer (stock), pH 7.4 (160 mM sodium phosphate, 4 M NaCl)? 100 ml? ? ? ?
2 M imidazole pH 7.4? 100 ml? ? ? ?

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*Analytical grade, >99% by HPLC analysis; all detergents are from Anatrace? Maumee, Ohio, USA (Affymetrix).
?Approximate critical micelle concentrations (CMC) in water at 20°C.

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TECHNICAL SPECIFICATIONS
His Mag Sepharose Ni
Matrix? Highly cross-linked spherical agarose (Sepharose) including magnetite?
Metal ion capacity? ~21 μmol Ni2+/ml medium?
Binding capacity? approximately 50 mg histidine-tagged protein/ml medium (~500 μg/purification run)?
Particle size? 37 to 100 μm?
Storage solution? 20% ethanol, 5% medium slurry?
Entire kit
Working temperature? Ambient?
Storage temperature? +2°C to +8°C?