Overview

Product name
Glutathione Fluorometric Detection Kit

See all Glutathione products (2) ...


Product overview
Glutathione Detection Kit provides a simple in vitro assay for detection of total glutathione changes in apoptosis and other samples. The assay utilizes monochlorobimane (MCB), a dye that appears to form an adduct exclusively with glutathione. The unbound MCB is almost nonfluorescent, whereas the dye fluoresces blue (Ex./Em. = 380nm/461nm) when bound to glutathione of reduced or oxidized form. The reaction is catalyzed by glutathione S-transferase. Thus, the amount of total glutathione can be easily detected using a fluorometer or a 96-well fluorometric plate reader.


Notes
After opening the kit, store MCB, GST, GSH at -20oC. Store Cell Lysis Buffer at 4oC for convenient use.


Tested applications
Functional Studiesmore details

Properties

Storage instructions
Store at -20°C, Stable for 6 months at -20°C



    

        

            
Components
            
Identifier
            
100 tests
        
    
    

        

            
Cell Lysis Buffer
            
WM
            
1 x 25ml
        
        

            
Glutathione Fluorometric Detection Kit
            
ab65322
            
1 x 100 kits
        
        

            
GSH Standard
            
Yellow
            
1 vial
        
        

            
GST Reagent
            
Green
            
1 x 200μl
        
        

            
Monochlorobimane
            
Red
            
1 x 200μl
        
    


http://www.abcam.com/Glutathione-Fluorometric-Detection-Kit-ab65322.html