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Species cross-reactivity is determined by western blot.

Applications Key:?W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry)

Specificity / Sensitivity

53BP1 Antibody detects endogenous levels of total 53BP1 protein independent of phosphorylation. 53BP1抗體檢測內源性獨立于磷酸化的53BP1總蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the center of human 53BP1. Antibodies are purified by protein A and peptide affinity chromatography. 該多克隆抗體由合成的人源的針對53BP1蛋白近中心區域殘基的肽段免疫動物，利用A蛋白和多肽親和層析的方法純化獲得。

Western Blotting

Western blot analysis of extracts from HT29 cells, using 53BP1 Antibody. Western blot 方法檢測HT29細胞提取物中53BP1蛋白水平，使用的抗體為53BP1 。

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using 53BP1 Antibody. 免疫組織化學方法檢測石蠟包埋的人乳腺癌組織，圖示為核定位。使用的抗體為53BP1 。

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma, using 53BP1 Antibody. 免疫組織化學方法檢測石蠟包埋的人腎細胞癌組織，使用的抗體為53BP1 。

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using 53BP1 Antibody in the presence of control peptide (left) or antigen-specific peptide (right). 免疫組織化學方法檢測石蠟包埋的人結腸癌組織，使用的抗體為53BP1 。左圖是對照組，右圖是抗原特異性肽段組。

IF-IC

Confocal immunofluorescent analysis of HeLa cells using 53BP1 Antibody (green). Actin filaments have been labeled with Alexa Fluor? 555 phalloidin (red). 激光共聚焦熒光法檢測HeLa 細胞，使用的抗體為53BP1(綠色)。肌動蛋白纖維采用Alexa Fluor? 555鬼筆環肽標記(紅色)。

Background

p53-binding protein 1 (53BP1) was originally identified as a p53 binding partner that could enhance the transcriptional activity of p53 (1,2). 53BP1 consists of two BRCA1 carboxy terminal (BRCT) domains that allow for binding to p53 and a separate domain responsible for binding to phosphorylated histone H2A.X (3). 53BP1 rapidly translocates to nuclear foci following treatment of cells with ionizing radiation (IR) or radiomimetic agents that cause DNA double strand breaks (DSBs) (4,5). Because of this localization to DSBs and homology to the yeast protein Rad9, a role for 53BP1 in DSB repair has been proposed. Recruitment of 53BP1 to sites of DNA damage has been demonstrated to be independent of ATM, NBS1, and DNA-PK (4) and retention of 53BP1 at DNA breaks requires phosphorylated H2A.X (6). In cells lacking 53BP1, phosphorylation of ATM substrates is reduced, suggesting that 53BP1 is upstream of ATM (7). In response to IR, phosphorylation of 53BP1 at serines 6, 25, 29, and 784 by ATM has been demonstrated, but phosphorylation at these sites is not required for localization of 53BP1 to sites of DSBs (6). p53結合蛋白1（p53 binding protein 1，53BPl）最初被認為是一種p53的結合蛋白，其功能是增強p53基因的轉錄活性。53BP1通過其兩個BRCA1羧基末端（BRCT）結構域與p53基因結合，通過另外一個獨立的結構域與磷酸化組蛋白H2A.X結合。電離輻射（IR）或者輻射類物預處理細胞后，可引起DNA雙鏈斷裂（DSBs），53BP1蛋白迅速轉移到核內。鑒于DNA斷裂后53BP1的定位改變及其與酵母蛋白Rad9的同源性，人們提出53BP1可能在DNA雙鏈斷裂后的修復過程中起到一定的作用。53BP1聚集到DNA損傷處的過程不依賴于ATM,NBS1,和DNA-PK，但是其在DNA斷裂處的滯留依賴于磷酸化H2A.X。在53BP1表達缺失的細胞中，ATM底物的磷酸化程度降低，提示53BP1在ATM上游起作用。電離刺激后，53BP1在絲氨酸6,25,29以及784位被ATM磷酸化，但是這些位點的磷酸化并不影響53BP1在DNA雙鏈斷裂處的聚集。

12630 SignalFire? Plus ECL Reagent

12757 SignalFire? Elite ECL Reagent

2524 p53 (1C12) Mouse mAb

2577 Phospho-Histone H2A.X (Ser139) Antibody

2661 Phospho-Chk2 (Thr68) Antibody

2674 Phospho-53BP1 (Ser25/29) Antibody

2675 Phospho-53BP1 (Ser1778) Antibody

3002 p95/NBS1 Antibody

3428 Phospho-53BP1 (Thr543) Antibody

6209 Phospho-53BP1 (Ser1618) (D4H11) Rabbit mAb

6883 SignalFire? ECL Reagent

7074 Anti-rabbit IgG, HRP-linked Antibody

7720 Prestained Protein Marker, Broad Range (Premixed Format)

7727 Biotinylated Protein Ladder Detection Pack