<!--ewebeditor:page title=""--> <p class="MsoNormal" style="font-size: medium;" align="left"><strong><span style="font-size: 14pt; color: #2e74b5;">產品信息</span></strong></p> <div style="font-size: medium;" align="center"> <table class="1" style="border-collapse: collapse; border: none;" border="1" cellspacing="0" cellpadding="0"> <tbody> <tr> <td style="width: 98.75pt; border: 1pt solid windowtext; background: #9cc2e5; padding: 0cm 5.4pt;" valign="top" width="132"> <p class="MsoNormal" align="left"><span style="font-size: 12pt;">產品貨號</span></p> </td> <td style="width: 212.6pt; border-top: 1pt solid windowtext; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-image: initial; border-left: none; background: #9cc2e5; padding: 0cm 5.4pt;" valign="top" width="283"> <p class="MsoNormal" align="left"><span style="font-size: 12pt;">產品名稱</span></p> </td> <td style="width: 164.05pt; border-top: 1pt solid windowtext; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-image: initial; border-left: none; background: #9cc2e5; padding: 0cm 5.4pt;" valign="top" width="219"> <p class="MsoNormal" align="left"><span style="font-size: 12pt;">產品規格</span></p> </td> </tr> <tr> <td style="width: 98.75pt; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-left: 1pt solid windowtext; border-image: initial; border-top: none; padding: 0cm 5.4pt;" valign="top" width="132"> <p class="MsoNormal" align="left"><span lang="EN-US" style="font-size: 11pt;"><span style="font-size: 12pt;">JY03061/JY03062</span></span></p> </td> <td style="width: 212.6pt; border-top: none; border-left: none; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; padding: 0cm 5.4pt;" valign="top" width="283"> <p class="MsoNormal" align="left"><span style="font-size: 12pt;">全套第二代慢病毒包裝質粒（三質粒系統）</span></p> </td> <td style="width: 164.05pt; border-top: none; border-left: none; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; padding: 0cm 5.4pt;" valign="top" width="219"> <p class="MsoNormal" align="left"><span lang="EN-US" style="font-size: 12pt;">1 &mu;g/100 &mu;g per plasmid</span></p> </td> </tr> </tbody> </table> </div> <p class="MsoNormal" style="font-size: medium;" align="left"><strong><span style="font-size: 14pt; color: #2e74b5;">&nbsp;</span></strong></p> <p class="MsoNormal" style="font-size: medium;" align="left"><strong><span style="font-size: 14pt; color: #2e74b5;">產品簡介</span></strong></p> <p class="MsoNormal" style="font-size: medium; text-indent: 21pt;">江苑生物的全套第二代慢病毒包裝質粒（三質粒系統）包括兩種包裝輔助質粒：<span lang="EN-US">pMD2.G</span>和<span lang="EN-US">psPAX2</span>（或<span lang="EN-US">pCMV-dR8.91</span>和<span lang="EN-US">pCMV-VSV-G</span>），均為氨芐青霉素抗性，用于包裝第二代和第三代慢病毒載體質粒。</p> <p class="MsoNormal" style="font-size: medium; text-indent: 21pt;">此包裝質粒系統表達病毒包裝需要的各種必需成分如：<span lang="EN-US">gag</span>基因，編碼病毒的核心蛋白如基質蛋白、衣殼蛋白和核衣殼蛋白等；<span lang="EN-US">pol</span>基因，編碼病毒復制所需的酶如反轉錄酶、整合酶和蛋白酶；<span lang="EN-US">rev</span>基因，編碼調節<span lang="EN-US">gag</span>和<span lang="EN-US">pol</span>基因表達的調節因子；還含有單純皰疹病毒來源的<span lang="EN-US">VSV-G</span>基因，提供病毒包裝所需要的包膜蛋白。江苑生物的慢病毒包裝系統產生的慢病毒病毒感染目的細胞后不會再感染其他細胞，也不會利用宿主細胞產生新的病毒顆粒。慢病毒中的毒性基因已經被剔除并被外源性目的基因所取代，屬于假型病毒。本司還提供<strong>慢病毒包裝試劑盒（江苑生物</strong><strong> <span lang="EN-US">JY03021</span></strong><strong>），包含全套的包裝組分和試劑。為了加速科研進程，本司還提供細胞基因過表達、</strong><strong><span lang="EN-US">shRNA/siRNA</span></strong><strong>介導的基因沉默、</strong><strong><span lang="EN-US">CRISPR/Cas9</span></strong><strong>介導的基因敲除等服務。</strong></p> <p class="MsoNormal" style="font-size: medium; text-indent: 21pt;"><strong>慢病毒包裝原理、包裝質粒的選擇、包裝系統和包裝步驟請參考：</strong><strong><span style="font-size: 12pt;">江苑生物</span><span lang="EN-US" style="font-size: 12pt;">&rarr;</span><span style="font-size: 12pt;">新聞中心</span></strong></p> <p class="MsoNormal" style="font-size: medium;" align="left"><strong><span style="font-size: 14pt; color: #2e74b5;">產品組分</span></strong></p> <div style="font-size: medium;" align="center"> <table class="MsoTableGrid" style="border-collapse: collapse; border: none; height: 84px;" border="1" width="632" cellspacing="0" cellpadding="0"> <tbody> <tr style="height: 7.75pt;"> <td style="width: 155.45pt; border: 1pt solid windowtext; background: #9cc2e5; padding: 0cm 5.4pt; height: 41px;" rowspan="2" width="207"> <p class="MsoNormal" style="text-align: center;" align="center"><span style="font-size: 12pt;">組分名稱</span></p> </td> <td style="width: 70.85pt; border-top: 1pt solid windowtext; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-image: initial; border-left: none; background: #9cc2e5; padding: 0cm 5.4pt; height: 41px;" rowspan="2" width="94"> <p class="MsoNormal" style="text-align: center;" align="center"><span style="font-size: 12pt;">儲存</span></p> </td> <td style="width: 247.45pt; border-top: 1pt solid windowtext; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-image: initial; border-left: none; background: #9cc2e5; padding: 0cm 5.4pt; height: 15px;" colspan="2" width="330"> <p class="MsoNormal" style="text-align: center;" align="center"><span style="font-size: 12pt;">產品貨號</span><span lang="EN-US" style="font-size: 12pt;">/</span><span style="font-size: 12pt;">規格</span></p> </td> </tr> <tr style="height: 20pt;"> <td style="width: 120.5pt; border-top: none; border-left: none; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; background: #9cc2e5; padding: 0cm 5.4pt; height: 26px;" width="161"> <p class="MsoNormal" style="text-align: center;" align="center"><span lang="EN-US" style="font-size: 11pt;">JY03061</span></p> </td> <td style="width: 126.95pt; border-top: none; border-left: none; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; background: #9cc2e5; padding: 0cm 5.4pt; height: 26px;" width="169"> <p class="MsoNormal" style="text-align: center;" align="center"><span lang="EN-US" style="font-size: 11pt;"><span style="font-size: 12pt;">JY03062</span></span></p> </td> </tr> <tr style="height: 15.7pt;"> <td style="width: 155.45pt; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-left: 1pt solid windowtext; border-image: initial; border-top: none; padding: 0cm 5.4pt; height: 21px;" width="207"> <p class="MsoNormal" style="text-align: center;" align="center"><span lang="EN-US" style="font-size: 12pt;">pMD2.G (or </span><span lang="EN-US" style="font-size: 12pt;">pCMV-dR8.9</span><span lang="EN-US" style="font-size: 12pt;">)</span></p> </td> <td style="width: 70.85pt; border-top: none; border-left: none; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; padding: 0cm 5.4pt; height: 21px;" width="94"> <p class="MsoNormal" style="text-align: center;" align="center"><span lang="EN-US" style="font-size: 12pt;">-20℃</span></p> </td> <td style="width: 120.5pt; border-top: none; border-left: none; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; padding: 0cm 5.4pt; height: 21px;" width="161"> <p class="MsoNormal" style="text-align: center;" align="center"><span lang="EN-US" style="font-size: 12pt;">1 &mu;g</span></p> </td> <td style="width: 126.95pt; border-top: none; border-left: none; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; padding: 0cm 5.4pt; height: 21px;" width="169"> <p class="MsoNormal" style="text-align: center;" align="center"><span lang="EN-US" style="font-size: 12pt;">100 &mu;g</span></p> </td> </tr> <tr style="height: 15.65pt;"> <td style="width: 155.45pt; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-left: 1pt solid windowtext; border-image: initial; border-top: none; padding: 0cm 5.4pt; height: 21px;" width="207"> <p class="MsoNormal" style="text-align: center;" align="center"><span lang="EN-US" style="font-size: 12pt;">psPAX2 (or </span><span lang="EN-US" style="font-size: 12pt;">pCMV-VSV-G</span><span lang="EN-US" style="font-size: 12pt;">)</span></p> </td> <td style="width: 70.85pt; border-top: none; border-left: none; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; padding: 0cm 5.4pt; height: 21px;" width="94"> <p class="MsoNormal" style="text-align: center;" align="center"><span lang="EN-US" style="font-size: 12pt;">-20℃</span></p> </td> <td style="width: 120.5pt; border-top: none; border-left: none; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; padding: 0cm 5.4pt; height: 21px;" width="161"> <p class="MsoNormal" style="text-align: center;" align="center"><span lang="EN-US" style="font-size: 12pt;">1 &mu;g</span></p> </td> <td style="width: 126.95pt; border-top: none; border-left: none; border-bottom: 1pt solid windowtext; border-right: 1pt solid windowtext; padding: 0cm 5.4pt; height: 21px;" width="169"> <p class="MsoNormal" style="text-align: center;" align="center"><span lang="EN-US" style="font-size: 12pt;">100 &mu;g</span></p> </td> </tr> </tbody> </table> </div> <p class="MsoNormal" style="font-size: medium;">注：<span lang="EN-US">pMD2.G</span>和<span lang="EN-US">psPAX2</span>較為常用，默認發此套質粒。</p> <p class="MsoNormal" style="font-size: medium;">&nbsp;</p> <p class="MsoNormal" style="font-size: medium;"><strong><span style="font-size: 14pt; color: #2e74b5;">保存條件</span></strong></p> <p class="MsoNormal" style="font-size: medium;">干冰運輸。<span lang="EN-US">-20℃</span>保存，避免反復凍融。</p> <p class="MsoNormal" style="font-size: medium;">&nbsp;</p> <p class="MsoNormal" style="font-size: medium;" align="left"><strong><span style="font-size: 14pt; color: #2e74b5;">注意事項</span></strong></p> <p class="MsoNormal"><span class="fontstyle11"><span style="font-size: medium;">1</span><span style="font-size: medium;">．</span><span style="font-size: 12pt;">質粒濃度由</span></span><span lang="EN-US" style="font-size: medium;">Thermo</span><span style="font-size: medium;">的</span><span lang="EN-US" style="font-size: medium;">NANODROP ONE</span><span style="font-size: medium;">測定，不同的儀器測出的濃度可能會有輕微差異。</span></p> <p class="MsoNormal" style="font-size: medium;"><span lang="EN-US">2</span>．本產品僅用于科學研究。</p> <p class="MsoNormal" style="font-size: medium;" align="left"><span lang="EN-US">3</span>．為了您的安全和健康，請穿實驗服并戴一次性手套操作。</p> <p class="MsoNormal" style="font-size: medium;" align="left">&nbsp;</p> <p class="MsoNormal" style="font-size: medium;" align="left"><strong><span style="font-size: 14pt; color: #2e74b5;">使用說明</span></strong></p> <p class="MsoNormal" style="font-size: medium;"><span lang="EN-US">1</span>．本品為高純度、無內毒素、無污染物的質粒，溶于<span lang="EN-US">ddH₂O</span>中。其中<span lang="EN-US">1 &mu;g</span>包裝的質粒濃度為<span lang="EN-US">100 ng/&mu;L</span>，共<span lang="EN-US">10 &mu;L</span>；<span lang="EN-US">100 &mu;g</span>包裝的質粒濃度為<span lang="EN-US">1000 ng/</span><span lang="EN-US">&mu;L</span>，共<span lang="EN-US">100 &mu;L</span><span style="background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">，使用時根據實際需求進行稀釋，分裝凍存</span>。</p> <p class="MsoNormal" style="font-size: medium;"><span class="fontstyle11"><span lang="EN-US" style="font-size: 9pt;">2</span></span><span class="fontstyle11"><span style="font-size: 9pt;">．若購買</span></span><span class="fontstyle11"><span lang="EN-US" style="font-size: 9pt;">1 </span></span><span lang="EN-US">&mu;g</span><span style="background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">包裝的質粒，</span><span class="fontstyle11"><span style="font-size: 9pt;">首次使用時請先取少量本質粒轉化大腸桿菌，進行質粒小量、中量或大量抽提后再用于后續用途。抽提獲得的質粒可以通過電泳或測序進行鑒定。若想省去擴增和鑒定步驟，可直接購買</span></span><span class="fontstyle11"><span lang="EN-US" style="font-size: 9pt;">100 </span></span><span lang="EN-US">&mu;g</span><span class="fontstyle11"><span style="font-size: 9pt;">包裝的質粒。</span></span></p> <p class="MsoNormal" style="font-size: medium;"><span lang="EN-US">3</span>．<span lang="EN-US">pMD2.G</span>、<span lang="EN-US">psPAX2</span>及慢病毒載體質粒，構成三質粒系統（即第二代慢病毒系統），共同轉染<span lang="EN-US">293T</span>細胞等即可以包裝慢病毒。</p> <p><strong><span style="color: #2e74b5;">相關產品</span></strong></p> <table width="643"> <tbody> <tr> <td width="161"> <p><span style="color: black;">產品編號</span></p> </td> <td width="321"> <p><span style="color: black;">產品名稱</span></p> </td> <td width="161"> <p><span style="color: black;">產品規格</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03011/JY03012</span></p> </td> <td width="321"> <p>慢病毒濃縮試劑盒</p> </td> <td width="161"> <p><span style="color: black;">50 mL/100 mL</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03013/JY03014</span></p> </td> <td width="321"> <p>PEG-8000慢病毒濃縮液</p> </td> <td width="161"> <p><span style="color: black;">50 mL/100 mL</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03061/JY03062</span></p> </td> <td width="321"> <p>全套第二代慢病毒包裝質粒（三質粒系統）</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03063/JY03064</span></p> </td> <td width="321"> <p>全套第三代慢病毒包裝質粒（四質粒系統）</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03027/JY03028</span></p> </td> <td width="321"> <p>慢病毒包裝質粒 pMD2.G</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03029/JY03030</span></p> </td> <td width="321"> <p>慢病毒包裝質粒 psPAX2</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03031/JY03032</span></p> </td> <td width="321"> <p>慢病毒包裝質粒 pCMV-VSV-G</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03033/JY03034</span></p> </td> <td width="321"> <p>慢病毒包裝質粒 pCMV-dR8.91</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03035/JY03036</span></p> </td> <td width="321"> <p>慢病毒包裝質粒 pMDLg/pRRE</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03037/JY03038</span></p> </td> <td width="321"> <p>慢病毒包裝質粒 pRSV-Rev</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY10011</span></p> </td> <td width="321"> <p>pLKO.1 puro</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY10012</span></p> </td> <td width="321"> <p>pLKO.1-EGFP-Puro</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY10013</span></p> </td> <td width="321"> <p>pLVX-Puro</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY10014</span></p> </td> <td width="321"> <p>pLenti-puro</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03041</span></p> </td> <td width="321"> <p>293T，HEK-293T，人胚腎細胞</p> </td> <td width="161"> <p><span style="color: black;">&gt; 1&times;10? cells</span></p> </td> </tr> </tbody> </table> <p class="MsoNormal" style="font-size: medium;">&nbsp;</p>