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DIG高效DNA標記及檢測Starter Kit 1
 
DIG High Prime DNA Labeling and Detection Starter Kit 1：Kit for color detection with NBT/BCIP
 
Roche公司現為用戶提供一種新的DIG標記及檢測試盒DIG High Prime DNA Labeling and Detection Kit I。與原有的DIG DNA Labeling and Detection kit(Cat.No.1093657)比較，本產品有以下特點：

●能對10×10cm印跡膜作12個標記反應及24個顯色檢測反應，是DIG DNA Labeling and Detection Kit(Cat.No.1093657)份量的一半，所以特別適合初用者及需處理較少量樣品的用戶使用。
●試劑盒采用的是DIG高效標記試劑(Cat.No.1585606)，其標記效率是標準DIG隨機引物標記法的3-10倍，而且預混了標記需要的所有試劑，即開即用，令操作更簡單和方便。
●試劑盒中還包括了DIG定量/對照試紙條，令您能在30分鐘內只用小量試劑便可估計DIG標記探針的濃度，方便您取得理想的信噪比。
●用于顯色的NBT/BCIP底物為預混的濃縮度，而封閉液則以10倍濃度形式供應，簡化了操作程序。
 
產品號
 產品規格
 廠商
 價格
 
1745832

11745832910
 Kit：1 kit (12 labeling reactions and 24 detection reactions)
 Roche
 4719
 

DIG-High Prime DNA Labeling and Detection Starter Kit I

Application

DIG-High Prime labeled probes can be used in Southern-, northern- and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and color detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering a ready-made blocking solution, combined stock solution of NBT/ BCIP, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.

 

Benefits

The High Prime method of labeling offers many benefits. Plasmid, cosmid, and lambda phage DNA have all been used as templates. We have achieved equal efficiency when labeling 10 ng to 3 g of template DNA, which may be linear or supercoiled.

 

Product Description

Sensitivity and specificity: The standard assay uses 1 g of linearized pBR328. It is usual to expect 0.8 g labeled DNA after 1 hour of labeling, and 2.3 g labeled DNA after a 20-hour reaction. In a dot-blot hybridization, 0.1 pg of homologous DNA is detectable after a 16-hour color development (probe concentrations at 20 ng/ml). A single-copy human gene (tPA) is detectable in a Southern blot, loading 1 g of digested placenta DNA.

 

Contents

1.       DIG-High Prime, 5x conc., 50 l 

2.       DIG-labeled Control DNA, 20 l, (5 g/ml), pBR328 (linearized with Bam HI) 

3.       DNA Dilution Buffer, 3 x 1 ml 

4.       Anti-digoxigenin-AP Conjugate, 100 l 

5.       NBT/BCIP Stock Solution, conc., 6 x 1 ml 

6.       Blocking Solution, 10x conc., 4 x 100 ml 

DIG Easy Hyb Granules, 4 x 100 ml