AKR1D1 monoclonal antibody (M03), clone 1C2
產品名稱： AKR1D1 monoclonal antibody (M03), clone 1C2
英文名稱： AKR1D1 monoclonal antibody (M03), clone 1C2
產品編號： H00006718-M03
產品價格： null
產品產地： 臺灣
品牌商標： Abnova
更新時間： null
使用范圍：
亞諾法生技股份有限公司（Abnova)
- 聯系人 :
- 地址 : 臺灣臺北市內湖區洲子街 108 號 9 樓
- 郵編 : 11493
- 所在區域 : 臺灣
- 電話 : +886-920**1152 點擊查看
- 傳真 : 點擊查看
- 郵箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant AKR1D1.
- Immunogen:
- AKR1D1 (NP_005980, 227 a.a. ~ 326 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- NPIWVNVSSPPLLKDALLNSLGKRYNKTAAQIVLRFNIQRGVVVIPKSFNLERIKENFQIFDFSLTEEEMKDIEALNKNVRFVELLMWRDHPEYPFHDEY
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG2b Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
- MSDS:
Download
- Applications
- Western Blot (Transfected lysate)
- Western Blot analysis of AKR1D1 expression in transfected 293T cell line by AKR1D1 monoclonal antibody (M03), clone 1C2.
Lane 1: AKR1D1 transfected lysate(37.4 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged AKR1D1 is approximately 0.3ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of AKR1D1 over-expressed 293 cell line, cotransfected with AKR1D1 Validated Chimera RNAi ( Cat # H00006718-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AKR1D1 monoclonal antibody (M03), clone 1C2 (Cat # H00006718-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 6718
- GeneBank Accession#:
- NM_005989
- Protein Accession#:
- NP_005980
- Gene Name:
- AKR1D1
- Gene Alias:
- 3o5bred,SRD5B1
- Gene Description:
- aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase)
- Omim ID:
- 604741
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. [provided by RefSeq
- Other Designations:
- aldo-keto reductase family 1, member D1,steroid 5-beta-reductase,steroid-5-beta-reductase, beta polypeptide 1 (3-oxo-5 beta-steroid delta 4-dehydrogenase beta 1)
- Related Disease