Anti-Active Ras Mouse Monoclonal Antibody
產品名稱： Anti-Active Ras Mouse Monoclonal Antibody
英文名稱： Anti-Active Ras Mouse Monoclonal Antibody
產品編號： 26909
產品價格： null
產品產地： 美國
品牌商標： NewEastbio
更新時間： null
使用范圍： IP, IHC
武漢紐斯特生物技術有限公司
- 聯系人 :
- 地址 : 武漢市東湖新技術開發區光谷生物城B3-3棟2-3樓
- 郵編 : 430074
- 所在區域 : 湖北
- 電話 : 186****7297 點擊查看
- 傳真 : 點擊查看
- 郵箱 : neweast3@126.com
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| Catalog Number:??26909 |
| Gene Symbol:??RAS |
| Description: ??Anti-Active Ras Mouse Monoclonal Antibody |
| Background: ??Small GTPases are a super-family of cellular signaling regulators. Ras belongs to the Ras sub-family of GTPases that regulate cell proliferation, cell motility, and gene transcription. GTP binding increases the activity of Ras, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). |
| Immunogen: ??Recombinant full length protein of active H-Ras |
| Applications: ??IP, IHC |
| Recommended Dilutions: ??1 μg for 1~2 mg total cellular proteins for IP; 1:100 dilution for IHC |
| Concentration: ??1 mg/ml |
| Host: ??mouse |
| Format: ??Liquid |
| Clonality: ??Monoclonal |
| Isotype: ??IgG |
| Purity: ??Purified from ascites |
| Storage buffer: |
| Preservative:??no |
| Constituents:??PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity:??Anti-active Ras antibody recognizes active H-Ras, N-Ras, and K-Ras from vertebrates. |
| Storage Conditions:??Store at -20°C. Avoid freeze / thaw cycles |
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| Immunoprecipitation/Western blot:?? |
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IP/WB analysis of active Ras protein. Purified recombinant Ras proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-active Ras mouse monoclonal antibody (Cat. # 26909). After SDS/PAGE, the membrane filter was probed with anti-Ras mouse monoclonal antibody (Cat. # 26014). |
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| Publications: |
| 1.??DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model ????Gene Therapy 21, 888-896 (October 2014) |