Product: Anti-3-Nitrotyrosine, clone 39B6
Cat. No.: MC-374 (50 μg)
Synonyms:
3NTyr
Specificity:
Recognizes 3-nitrotyrosine moieties.
Species Reactivity:
Not species specific.
Ig Isotype:
Mouse IgG2a
Immunogen:
3-(4-Hydroxy-3-nitrophenylacetamido) propionic
acid-BSA conjugate.
Format:
50 μg of purified antibody at 0.5 mg/mL in PBS
with 0.09 sodium azide.
Storage:
Store at 4°C short term and at -20°C long term.
Aliquot to avoid freeze/thaw cycles.
Applications and Suggested Dilutions:

ELISA: With antigen coated plates

Immunohistochemistry: Frozen and paraffin
sections

Immunoprecipitation

Western blots: Use at a 1:5,000 dilution.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.
Lane 1: 0.5 μg BSA
Lane 2: 0.1 μg Nitrotyrosine-modified BSA
MC-374 was used at a 1:5,000 dilution. Secondary antibody
(HRP-conjugated anti-mouse IgG) used at a1:5,000 dilution