Recombinant Human EGF Protein (rp145392)-Protein Bioactivity
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9ng/mL.
Recombinant Human EGF Protein
產品名稱： Recombinant Human EGF Protein
英文名稱： Recombinant Human EGF Protein
產品編號： rp145392
產品價格： 0
產品產地： 上海
品牌商標： 阿拉丁
更新時間： 2023-08-04T14:27:25
使用范圍： null
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基本信息
| 產品名稱 | Recombinant Human EGF Protein |
|---|---|
| 英文名稱 | Recombinant Human EGF Protein |
| 規格或純度 | GMP, >95% SDS-PAGE, Active,Pichia pastoris, No tag, 971-1021aa |
| 生物活性 | Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9 ng/mL. |
| 內毒素水平 | < 0.2 EU/µg |
| 表達系統 | Pichia pastoris |
| 種屬 | Human |
| 氨基酸 | 971-1021 aa |
| 序列 | NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWW ELR |
| 蛋白標簽 | No tag |
| N-末端序列 | NSDSECPLSH |
| 蛋白長度 | Full length protein |
| Carrier Free | Yes |
| AnimalFree | Yes |
| Accession# | P01133 |
| 來源 | Recombinant |
| 預測分子量 | 6 kDa |
| SDS-PAGE | 6.6 kDa, under reducing conditions. |
相關屬性
| 特點與好處 | ·Application:Cell Culture,FuncS,HPLC,MS,SDS-PAGE |
|---|---|
| 物理外觀 | Lyophilized |
| 儲存緩沖液 | 10mM磷酸鈉緩沖液 |
| 復溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 10mM PBS to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 |
| 儲存溫度 | -20°C儲存 |
| 運輸條件 | 超低溫冰袋運輸 |
| 穩定性與儲存 | 在-20℃下穩定至 24 個月，在 2~8℃下穩定至 4 周。配制為 100μg/ml 的儲存液，2~8℃可穩定保存一周。若需延長儲存時間，建議在-70℃或-20℃冷凍，不建議重復冷凍和解凍。 |
常規信息
| 基因名 | EGF - epidermal growth factor |
|---|---|
| 基因 ID | 1950 |
| 細胞定位 | Membrane, Secreted |
| 序列相似性 | Contains 9 EGF-like domains. Contains 9 LDL-receptor class B repeats. |
| 涉及疾病 | Hypomagnesemia 4 |
| 基因別名 | HOMG4, URG |
| 英文別名 | beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; hEGF; HOMG4; pro-epidermal growth factor; URG; Urogastrone |
| 別名 | 重組人表皮生長因子蛋白(EGF)；beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; hEGF; HOMG4; pro-epidermal growth factor; URG; Urogastrone |
一般描述
Purity
≥ 95% SDS-PAGE. ≥ 95 % HPLC.
Additional sequence information
N-terminal glycine. Full-length mature EGF chain.
Function
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
Post-translational
O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
圖片
Recombinant Human EGF Protein (rp145392)-SDS-PAGE
5μg/lane of Recombinant Human EGF was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 6.6 kDa.