• 特異性ab195847 detects endogenous levels of Caspase-9 only when phosphorylated at Threonine 125.
• 經測試應用WBmore details
• 種屬反應性
  與反應:?Mouse, Rat, Human
• 免疫原

  Synthetic peptide corresponding to Human Caspase-9 (phospho T125). A synthesized peptide derived from Human Caspase-9 around the phosphorylation site of Threonine 125.
  Database link:?P55211

• 陽性對照
  • TNF treated HeLa whole cell lysates.

性能

• 形式Liquid
• 存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存儲溶液pH: 7.4
  Preservative: 0.02% Sodium azide
  Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol
• 濃度100 μg 濃度為 1 mg/ml?
  
• 純度Immunogen affinity purified
• 克隆多克隆
• 同種型IgG
• 研究領域
  • Cell Biology
  ?
  • Apoptosis
  ?
  • Intracellular
  ?
  • Caspases etc
  ?
  • Caspases

  • Cancer
  ?
  • Invasion/microenvironment
  ?
  • Apoptosis
  ?
  • Caspases

  • Cancer
  ?
  • Invasion/microenvironment
  ?
  • Apoptosis
  ?
  • Cytochrome C

  • Metabolism
  ?
  • Pathways and Processes
  ?
  • Metabolism processes
  ?
  • Apoptosis

  • Cancer
  ?
  • Cell Death
  ?
  • Apoptosis
  ?
  • Apoptotic Markers
  ?
  • Caspases

  • Cancer
  ?
  • Cell Death
  ?
  • Apoptosis
  ?
  • Apoptotic Markers
  ?
  • Cytochrome C

  • Cancer
  ?
  • Cell Death
  ?
  • Apoptosis
  ?
  • Metabolism

應用

靶標

• 功能Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
  Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
• 組織特異性Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
• 序列相似性Belongs to the peptidase C14A family.
  Contains 1 CARD domain.
• 發展階段Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
• 翻譯后修飾Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.

• Anti-Caspase-9 (phospho T125) antibody 圖像

  • 

    Western blot - Anti-Caspase-9 (phospho T125) antibody (ab195847)
    All lanes :?Anti-Caspase-9 (phospho T125) antibody (ab195847) at 1/500 dilution
    
    Lane 1 :?TNF treated HeLa whole cell lysates with antigen-specific peptide
    Lane 2 :?TNF treated HeLa whole cell lysates
    
    Lysates/proteins at 12.5 μg per lane.
    
    
    Predicted band size :?46 kDa