Disease:

 Cervical carcinoma 

Origin:

 These cells were established from cervical cancer biopsies. 

Species:

Homo sapiens 

Tissue:

Cervix 

Morphology:

Epithelial 

Properties:

Adherent 

Cytogenic data:

Pseudodiploid human cell line 

Patient:

Female, Caucasian, 66 yrs of age 

Medium:

EMEM + 10% FBS 

Subculture:

1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:

I 

Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative 

Isoenzymes:
 AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 2; PGM1, 1; PGM3, 1 
DNA Profile (STR):
 Amelogenin: X CSF1PO: 12 D13S317: 13 D16S539: 13,14 D5S818: 11,12 D7S820: 10 THO1: 7,8 TPOX: 9 vWA: 18,20 
Related cells by Organ:

HEC-59 cells 

HeLa cells 

SiHa cells 

CaSKi cells 

C33A cells 


References for C33A cells:

1. Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985.