Size??100ug
Cat code??200784
Species Cross-reactivity??H,M,R,Mk
Key Application??WB,IP
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG2b
Molecular Weight??55kDa
Sensitivity??This antibody detects endogenous levels of RAD9A,and does not cross-react with related proteins.
Immunogen??Purified recombinant human Rad9A protein fragments expressed in E.coli
UniProt Number??Q99638
GeneBank ID??NM_004584
Subcellular location??Nucleus
Formulation??Purified mouse monoclonal in PBS(pH 7.4)containing with 0.2% sodium azide,50% glycerol.
Altername??Cell cycle checkpoint control protein;DNA repair exonuclease rad9 homolog A;hRAD 9;hRAD9;Rad 9;RAD 9A;RAD9 (S pombe) homolog;RAD9 homolog A;RAD9 homolog;RAD9A;
Gene_symbol??RAD9
Summary??This gene product is highly similar to Schizosaccharomyces pombe rad9,a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair.This protein possesses 3′ to 5′ exonuclease activity,which may contribute to its role in sensing and repairing DNA damage.It forms a checkpoint protein complex with RAD1 and HUS1.This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage,which is thought to be important for triggering the checkpoint-signaling cascade.Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq,Aug 2011]
UniPort summary  Function??Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair.The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C(RFC)clamp loader complex.Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair(LP-BER).The 9-1-1 complex stimulates DNA polymerase beta(POLB)activity by increasing its affinity for the 3′-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds;endonuclease FEN1 cleavage activity on substrates with double,nick,or gap flaps of distinct sequences and lengths;and DNA ligase I(LIG1)on long-patch base excision repair substrates.The 9-1-1 complex is necessary for the recruitment of C12orf32/RHINO to sites of double-stranded breaks(DSB)occurring during the S phase.RAD9A possesses 3′->5′ double stranded DNA exonuclease activity.Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Application Images :??
Western blot detection of RAD9A in Hela,MCF7,3T3,COS7&Rat testis cell lysates using RAD9A antibody(1:500 diluted).Predicted band size:43KDa,Observed band size:55KDa
Immunoprecipitation analysis of Hela & 3T3 cell lysates using RAD9A antibody