Active RhoA GTP [30 µL]

Catalog Number:  CR-M26904

Gene Symbol:  RHOA

Description:   Anti Active RhoA Mouse Monoclonal Antibody

Background:   Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca 2+ sensitivity of smooth muscle contraction.

Immunogen:   Recombinant full length protein of active RhoA

Applications:   IP, IHC

Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 1:100 dilution for IHC

Concentration:   1 mg/ml

Host:   mouse

Format:   Liquid

Clonality:   Monoclonal

Isotype:   IgG2b

Purity:   Purified from ascites

Storage buffer:

Preservative:  no

Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity:  Anti-active RhoA antibody recognizes active RhoA from vertebrates.

Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles

IP/WB analysis of active RhoA protein. Purified recombinant RhoA proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitatedwith anti active RhoA mouse monoclonal antibody (Cat. # CR-M26904). After SDS/PAGE, themembrane filter was probed with anti RhoA mouse monoclonal antibody (Cat. # CR-M26007).