All Names: TBB2, TBB5, TUBB, TUBB5, Tubulin beta chain, Tubulin beta-5 chain
Form of Antibody: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability: Store at -20℃/1 year?
Clonality: Polyclonal?
Immunogen: The antiserum was produced against synthesized peptide derived from C-terminal of human Tubulin β.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity/Sensitivity: Tubulin β antibody detects endogenous levels of total Tubulin β protein.
Reactivity: Human, Mouse, Rat
Applications: WB: 1:500~1:3000 IHC: 1:50~1:100 ELISA: 1:5000
The optimal working dilution should be determined by the end user.
References: J E Rudolph, Mol. Cell. Biol., Jun 1987; 7: 2231 - 2242.
Jong-Soo Chang, J. Biol. Chem., Feb 2005; 280: 6897 - 6905.
Virginie Redeker, J. Biol. Chem., Jan 2005; 280: 596 - 606.
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WB:Western blot analysis of extracts from rat cells and mouse brain cells, using Tubulin β antibody.
The lane on the right is treated with the synthesized peptide.