Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. The EZ DNA Methylation-Gold kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for downstream analyses, including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
HIGHLIGHTS：
Complete, high-throughput bisulfite conversion of GC-rich DNA in less than 3 hours.
A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines to uracil.
High throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA. Eluted, ultra-pure DNA is ideal for use in subsequent molecular-based analyses.