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Biotinylated Polyclonal Antibodies
Biotinylated Anti-Human ICAM-1





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		Preparation :
Produced from sera of rabbits immunized with highly pure recombinant Human ICAM-1.  Anti-Human ICAM-1 specific antibody was purified by affinity chromatography and then biotinylated.



		Catalog #:
500-P287Bt



		Source :
Rabbits



		Formulation :
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.



		Stability :
The lyophilized antibody is stable at room temperature for up to 1 month.  The reconstituted antibody is stable for at least two weeks at 2-8oC. Frozen aliquots are stable for at least 6 months when stored at -20oC. Avoid repeated freeze-thaw cycles.


Reconstitution:
Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.



		Applications :
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		Elisa?
Direct:To detect Human ICAM-1 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 C 1.0 μg/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 C 0.4 ng/well of recombinant Human ICAM-1.
 Sandwich:To detect Human ICAM-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 C 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human ICAM-1 (500-P287) as a capture antibody, allows the detection of at least 0.2 C 0.4 ng/well of recombinant Human ICAM-1.



		Western Blot?
To detect Human ICAM-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human ICAM-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.


Country of Origin :
USA
















        Size A
25μg
$195?
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        Size B
50μg
$250?
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        Size C
1mg
$4000?
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Special 
                    Sizes: 
 Many products can be ordered in custom sizes. Please click here to contact Peprotech with your request.





Usage:
For Research Use Only.  Not for use in diagnostic or therapeutic procedures.