Anti-MEK1 (I111S) Mouse Monoclonal Antibody-抗體-抗體-生物在線

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武漢紐斯特生物技術有限公司
Anti-MEK1 (I111S) Mouse Monoclonal Antibody

Anti-MEK1 (I111S) Mouse Monoclonal Antibody

商家詢價

產品名稱： Anti-MEK1 (I111S) Mouse Monoclonal Antibody

英文名稱： Anti-MEK1 (I111S) Mouse Monoclonal Antibody

產品編號： 26264

產品價格： null

產品產地： 美國

品牌商標： NewEastBio

更新時間： null

使用范圍： ELISA、WB、 IHC

武漢紐斯特生物技術有限公司
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MEK1 (I111S) [26264]
[100ul]		 ?

Catalog Number:??26264
Gene Symbol:??MAP2K1; MAPKK1; MEK1; MKK1; PRKMK1
Description: ??Anti-MEK1 (I111S) Mouse Monoclonal Antibody
Background: ??MEK1 (also known as extracellular signal-regulated kinases, ERKs) plays important role in the MAP kinase cascade, which transduces multiple extracellular signals to control cell growth, proliferation and differentiation. Activated upon growth factors stimulation, MEK1 phosphorylates MAPK3/ERK1 and MAPK1/ERK2, thus activates the MAP pathway and regulates transcription. Abnormal of the MEK1 protein, including point mutations, are implicated in diseases such as cardiofaciocutaneous syndrome (CFC) and melanoma.
Immunogen: ??A synthetic peptide from the internal region of MEK1 which includes the mutation of I111S, human origin.
Applications: ??ELISA, WB, IHC
Recommended Dilutions: ??
ELISA: 1:1000-1:5000?
WB: 1:500-1:1000?
IHC: 1:50-1:100
Concentration: ??1 mg/ml
Host: ??Mouse
Format: ??Liquid
Clonality: ??Monoclonal
Isotype: ??
Purity: ??Purified from ascites
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg2+?and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??Recognizes I111S mutant, but not wild-type MEK1 of vertebrates.
Storage Conditions:???Store at -20°C. Avoid freeze / thaw cycles
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Western blot:??
Western blot analysis of recombinant MEK1 (I111S) and wildtype proteins. Purified His-tagged MEK1 (I111S) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-MEK1 (I111S) monoclonal antibody (Cat. #26264).
Immunofluorescence: ??
Immunofluorescence of cells expressing MEK1 proteins with anti-MEK1 (I111S) antibody. HEK293T cells were transfected with pCDNA3-GFP-MEK1 (WT) plasmid (left column) or pCDNA3-GFP-MEK1 (I111S) plasmid (right column), then fixed and stained with anti-MEK1 (I111S) monoclonal antibody (Cat. #26264).