美國Optofluidic品牌nanotweezer微流控納米拉曼光鑷(NanoTweezer Raman)

—光譜分析進行個別納米粒子識別和化學分析(Individual Nanoparticle Identification and Chemical Analysis with Spectroscopy)
進行使用NanoTweezer拉曼光鑷化學分析納米顆粒液體樣品.

　微流控技術是在尺度為幾個或上百微米的通道中操縱納升或納升以下流體的技術。

光鑷拉曼光譜技術是將光學囚禁技術與顯微拉曼光譜技術相結合用于細胞或

其它生物大分子、病毒、蛋白的一項新技術本項目將激光光鑷拉曼技術與微流控芯片技術聯合起來構建了一套全自動化平臺系統。

美國Optofluidic成功的把微流控芯片技術與激光光鑷拉曼技術結合起來，并使流體速度得到了很好的控制，通過圖像識別的方式來判斷細胞是否被俘獲，通過程序來實現俘獲細胞、收集光譜、釋放細胞的完全自動化實驗操作，通過光譜我們可以很方便地區分正常的血紅細胞與地中海貧血的血紅細胞及其他納米級微粒。

通過圖像識別技術，實現了在微流控芯片的微通道里，由光鑷子將單個活細胞（其他納米粒子）或細胞器長時間固定在焦點附近，進行拉曼光譜分析。用拉曼光鑷子系統實時

監測單個活細胞或細胞器的生命過程，能夠在細胞和細胞器水平上，

為研究生命活動的本質提供重要信息

該NanoTweezer拉曼光鑷使研究人員能夠以前所未有的清晰，信號強度和精度來捕獲、可視化和獲取個別納米粒子拉曼光譜信息.
這種創新利用近場光散射法允許NanoTweezer拉曼光譜通過拉曼光譜獲取單個納米粒子的分子指紋。處于消逝場的形式增強信號和最小化背景噪聲，提供了遠遠優于比傳統的照明系統的結果。
拉曼信號通常非常弱，難以獲得。拉曼信號通常非常弱，難以獲得。傳統系統使用的傳統激光照射集中在一個塊狀片材料，粉末或溶液使得難以從背景中分離感興趣的材料并準確地表征它。到現在為止的拉曼系統只能被用于分析大量單粒子（幾微米），并在干燥的環境中。該NanoTweezer拉曼光譜消除這些障礙，使溶液中的單個納米化學識別成為現實。

Perform chemical analysis on a liquid sample of nanoparticles with the NanoTweezer Raman.
納米粒子個體（或小團體）都在使用我們的專利波導的方法暴露于激光，并獲得他們的拉曼光譜。這允許兩種類別分析:
1）未知微粒識別，例如，是否有問題的每個粒子是聚苯乙烯，玻璃，二氧化鈦，等等。
2）一種方法來監控用那些經過化學改性的藥物顆粒之間的差異，例如，裝載的納米顆粒，觀察降低而引起退化，化學修飾過程的質量控制。
特性列表?
1.對于包括質量控制，環境分析（納米粒子宿命），藥物遞送，納米毒性藥品和食品制劑研發的重點領域革命性變革(Revolutionary for key areas of R&D including quality control, environmental analysis (nanoparticle fate), drug delivery, nanotoxicity and drug and food formulations).
2.在其天然溶液環境中亞微米顆粒識別(Identification of sub-micron particles in their native solution environment).
3.使用小體積(<200μL)微流體流動池直截了當地處理樣品
4.用戶不必手動搜索粒子
5.近場光散射技術產生增強信號和最小背景噪音
NanoTweezer使用近場拉曼光譜?
不像其他的拉曼光譜顯微鏡，它只能提供關于微?；蜉^大的信息，該NanoTweezer可以捕集，可視化，獲得真正納米顆粒拉曼光譜光譜。而不是外部激光聚焦到底物上，它采用近場光泄漏出波導光學地激發和捕集在其天然環境中的顆粒。該強光漸逝場的形式導致升高的信號，并比傳統的照明系統背景更少。此外，由于粒子在分析期間被暫時捕獲，一個任意長曝光時間可以獲取。

背景?
Raman Spectroscopy??
Raman Spectroscopy is a powerful analytical?? technique that uses a laser to obtain a chemical?? signature from a material. The interrogating light (a?? single wavelength) interacts with the chemical bonds in?? the material causing an energy shift depending on the?? type of bond. These shifts show up as peaks in a?? Raman spectrum. The spectrum is unique to a?? material (glass, polystyrene, titanium dioxide, etc.) and?? can be considered a fingerprint
Raman Limitations
While Raman Spectroscopy can allow researchers to extract molecular fingerprints, Raman signals are typically very weak and difficult to?
obtain. Traditionally, a laser is focused on a bulk piece of material, powder, or solution making it harder to separate the material of interest?
from the background and characterize it accurately. Until now Raman systems could only be used to look at particles if they are stationary?
(not in solution) and visible (greater than a few microns).?
Near Field Raman Using the NanoTweezer
Unlike other Raman microscopes, which can only provide information about microparticles or larger, the NanoTweezer can trap, visualize,?
and obtain Raman spectra from true nanoparticles. Rather than an external laser focused onto a substrate, it uses near field light leaking?
out of a waveguide to optically excite and trap the particles in their native environments. This is the key breakthrough that enables the?
performance increase. The intense light in the form of an evanescent field leads to heightened signal and less background than traditional?
illumination systems. Additionally, because the particles are temporarily trapped during the analysis, an arbitrarily long exposure time can?
be acquired.

Includes

NanoTweezer with 785 nm laser
Microscope with NIR objectives
Microscope camera
Raman Spectrometer
Raman Software

The Benefits of Near-field Light Scattering

New Insights into Particle Analysis

Nanoparticle behavior and function is inextricably linked to the properties of its surface. Yet until today, no technique has allowed researchers to adequately characterize the properties of the surfaces of their nanoparticles in a detailed, simple and high throughput way. ?Near-field Light Scattering (NLS) enables all three of those by quickly mapping the energy and force landscapes of particle-surface and particle-particle interactions.?
Instead of just generating an overtly simplistic number (like zeta potential or debye length) NLS enables heightened information of these interactions which is key for ensuring proper function and designing stable formulations.?
A quick example to bring this home: properly coated particles allow strong repulsive forces, culminating in more stability and less aggregation.?
NLS is useful for key areas of R&D including drug delivery. In this field, particles need to be designed to be stable as well as perform a key specific function (like bind, block, regulate, etc.) Such design is done at the single particle level, and particles are expected to act on an individual basis rather than an aggregated basis, since aggregated particles either become ineffective or dangerous.?
One key benefit of NLS is that it allows researchers to analyze and distinguish between different aggregation states for the same particle population.

Comparison with State-of-the-Art Methods:

With a myriad of other particle analysis tools available, why use NLS?
Some key advantages include:?
1. Universal application

?