Size??100ug
Cat code??200765
Species Cross-reactivity??H
Key Application??WB,ICC
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG1
Molecular Weight??30kDa
Sensitivity??This antibody detects endogenous levels of CBR1,and does not cross-react with related proteins.
Immunogen??Purified recombinant human CBR1 protein fragments expressed in E.coli
UniProt Number??P16152
GeneBank ID??NM_001757
Subcellular location??Cytoplasm
Formulation??Purified mouse monoclonal in PBS(pH 7.4)containing with 0.2% sodium azide,50% glycerol.
Altername??15 hydroxyprostaglandin dehydrogenase [NADP+];15-hydroxyprostaglandin dehydrogenase [NADP+];Carbonyl reductase [NADPH] 1;Carbonyl Reductase 1;CBR 1;CBR1;CBR1_HUMAN;CRN;NADPH dependent carbonyl reductase 1;NADPH-dependent carbonyl reductase 1;Prostaglandin 9 ketoreductase;Prostaglandin 9-ketoreductase;Prostaglandin E(2) 9 reductase;Prostaglandin-E(2) 9-reductase;SDR21C1
Gene_symbol??CBR; hCBR1; SDR21C1
Summary??Carbonyl reductase is one of several monomeric,NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds.This enzyme is widely distributed in human tissues. Another carbonyl reductase gene,CRB3,lies close to this gene on chromosome 21q.[provided by RefSeq,Jul 2008]
UniPort summary  Function??NADPH-dependent reductase with broad substrate specificity.Catalyzes the reduction of a wide variety of carbonyl compounds including quinones,prostaglandins, menadione,plus various xenobiotics.Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha.Can bind glutathione,which explains its higher affinity for glutathione-conjugated substrates.Catalyzes the reduction of S-nitrosoglutathione.
Application Images :??
Western blot detection of CBR1 in Hela,A431 & MDA-MB-468 cell lysates using CBR1 antibody(1:1000 diluted).Predicted band size:30KDa,Observed band size:30KDa.
Immunocytochemistry stain of Hela using CBR1 antibody (1:100).