Anti-AKT1 Rabbit Polyclonal Antibody-抗體-抗體-生物在線

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武漢紐斯特生物技術(shù)有限公司
Anti-AKT1 Rabbit Polyclonal Antibody

Anti-AKT1 Rabbit Polyclonal Antibody

商家詢價

產(chǎn)品名稱： Anti-AKT1 Rabbit Polyclonal Antibody

英文名稱： Anti-AKT1 Rabbit Polyclonal Antibody

產(chǎn)品編號： 21274

產(chǎn)品價格： null

產(chǎn)品產(chǎn)地： 美國

品牌商標(biāo)： NewEastBio

更新時間： null

使用范圍： ELISA、WB、IHC

武漢紐斯特生物技術(shù)有限公司
  • 聯(lián)系人 :
  • 地址 : 武漢市東湖新技術(shù)開發(fā)區(qū)光谷生物城B3-3棟2-3樓
  • 郵編 : 430074
  • 所在區(qū)域 : 湖北
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  • 郵箱 : neweast3@126.com

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AKT1 [21274]
[100 μL]		 ?

Catalog Number:??21274
Gene Symbol:??AKT1, AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Description: ??Anti-AKT1 Rabbit Polyclonal Antibody
Background: ??AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. Its function is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. There are evidences that mutation or abnormal expression in AKT1 is associated with cancers.
Immunogen: ??Purified AKT1 protein, human origin.
Applications: ???ELISA, WB, IHC
Recommended Dilutions: ??
ELISA: 1:1000-1:5000?
WB: 1:100-1:1000?
IHC: 1:50-1:200
Concentration: ??0.2mg/ml
Host: ??Rabbit
Format: ??Liquid
Clonality: ??Polyclonal
Isotype: ??
Purity: ??Purified from serum
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg2+?and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??Recognizes AKT1 of vertebrates.
Storage Conditions:???Store at -20°C. Avoid freeze / thaw cycles
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Western blot:??
Western blot analysis of recombinant AKT1 protein. Purified His-tagged AKT1 protein was blotted with anti-AKT1 rabbit polyclonal antibody (Cat. #21274).
Immunohistochemistry:??
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with anti-AKT1 polyclonal antibody (Cat. #21274). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.