p38α MAPK (L53F8) Mouse-抗體-抗體-生物在線
杭州沃森生物技術有限公司
p38α MAPK (L53F8) Mouse

p38α MAPK (L53F8) Mouse

商家詢價

產品名稱： p38α MAPK (L53F8) Mouse

英文名稱： p38α MAPK (L53F8) Mouse

產品編號： mAb #9228

產品價格： null

產品產地： 美國

品牌商標： CST

更新時間： 2023-09-21T00:33

使用范圍：

杭州沃森生物技術有限公司
  • 聯系人 :
  • 地址 : 杭州市西溪路525號浙江大學科技園B座501室
  • 郵編 : 310029
  • 所在區域 : 浙江
  • 電話 : 137****2380 點擊查看
  • 傳真 : 點擊查看
  • 郵箱 : service@ws-bio.com

p38α MAPK (L53F8) Mouse mAb #9228

PhosphoSitePlus?protein, site, and accession data:?P38A
No.
Size
9228S 100 ul ( 10 western blots ) ?
9228 ? ?
Application
Dilution
Species-Reactivity
Sensitivity
MW (kDa)
Isotype
W 1:1000 Human,?Mouse,?Rat,?Monkey,?Pig,S. cerevisiae Endogenous 40 Mouse IgG1
IF-IC 1:25
F 1:200

Species cross-reactivity is determined by western blot.

Applications Key:?W=Western Blotting,?IF-IC=Immunofluorescence (Immunocytochemistry),?F=Flow Cytometry

?
Specificity / Sensitivity

p38α MAP Kinase (L53F8) Mouse mAb detects endogenous levels of total p38α MAPK. This antibody does not cross-react with either JNK/SAPK or p42/44 MAPK or other isoforms of p38.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant p38 MAPK protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells, using p38α MAPK (L53F8) Mouse mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, using p38α MAPK (L53F8) Mouse mAb (blue) compared to a nonspecifc negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or anisomycin-treated (right), using p38-alpha MAPK (L53F8) Mouse mAb (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Background

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).

SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).

Application References

Have?you?published research involving the use of our products? If so we'd love to hear about it. Please?let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Alexa Fluor?is a registered trademark of Molecular Probes, Inc.

Cell Signaling Technology?is a trademark of Cell Signaling Technology, Inc.