Product Highlight

• Configuration-specific monoclonal antibody that recognizes Rab5-GTP, but not Rab5-GDP
• The only available Rab5A activation assay kit directly measures the level of Rab5A-GTP
• Providing shorter experimental time
• Could be used to monitor the activation of Rab5 in cells and in tissues by immunohistochemistry

Product Description

Small GTPases are a super-family of cellular signaling regulators. Rab5 is a member of the Rab-family GTPases. Rab5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway.

Currently there is no direct assay to measure the activation of Rab GTPases.

NewEast Biosciences Rab5A Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab5-GTP, but not Rab5-GDP, and a Rab5A specific rabbit polyclonal antibody. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.

This anti-Rab5-GTP monoclonal antibody can also be used to monitor the activation of Rab5 in cells and in tissues by immunohistochemistry.

NewEast Biosciences Rab5A Activation Assay Kit provides a simple and fast method to monitor the activation of Rab5A. Each kit provides sufficient quantities to perform 20 assays.

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Assay Principle

NewEast Biosciences Rab5A Activation Assay Kit bases on the configuration-specific anti-Rab5-GTP monoclonal antibody to measure the active Rab5A-GTP levels, either from cell extracts or from in vitro GTP_γS loading Rab5A activation assays. Briefly, anti-active Rab5 mouse monoclonal antibody will be incubated with cell lysates containing Rab5-GTP. The bound active Rab5 will then be pulled down by protein A/G agarose. The precipitated active Rab5A will be detected by immunoblot analysis using anti- Rab5A specific rabbit polyclonal antibody.

Publications:

1.??VASP promotes TGF-β activation of hepatic stellate cells by regulating Rab11 dependent plasma membrane targeting of TGF-β receptors ????Hepatology Volume 61, Issue 1, pages 361–374, January 2015

2.??Rab5 Activity Regulates GLUT4 Sorting into Insulin-Responsive and Non-Insulin-Responsive Endosomal Compartments: A Potential Mechanism for Development of Insulin Resistance ????Endocrinology. 2014 Sep;155(9):3315-28