Anti-Active Rab35 Mouse Monoclonal Antibody
產品名稱： Anti-Active Rab35 Mouse Monoclonal Antibody
英文名稱： Anti-Active Rab35 Mouse Monoclonal Antibody
產品編號： 26922
產品價格： null
產品產地： 美國
品牌商標： NewEastbio
更新時間： null
使用范圍： IP, IHC,IF
武漢紐斯特生物技術有限公司
- 聯系人 :
- 地址 : 武漢市東湖新技術開發區光谷生物城B3-3棟2-3樓
- 郵編 : 430074
- 所在區域 : 湖北
- 電話 : 186****7297 點擊查看
- 傳真 : 點擊查看
- 郵箱 : neweast3@126.com
| Catalog Number:??26922 |
| Gene Symbol:??RAB35 |
| Description: ??Anti-Active Rab35 Mouse Monoclonal Antibody |
| Background: ??The Rab family of GTPases regulates eukaryotic vesicular membrane traffic. Rab35 is with both plasma membrane and endosomal localization, and has been implicated in diverse processes that include T-cell receptor recycling, oocyte yolk protein recycling and cytokinesis. Rab35 regulates neurite outgrowth in neuronal-like cells, and can induce protrusions. |
| Immunogen: ??Recombinant full length protein of active Rab35 |
| Applications: ??IP, IHC, IF |
| Recommended Dilutions: ?? 1 μg for ~2 mg total cellular proteins for IP 1:100 dilution for IHC, IF |
| Concentration: ??1 mg/ml |
| Host: ??mouse |
| Format: ??Liquid |
| Clonality: ??Monoclonal |
| Isotype: ??IgG |
| Purity: ??Purified from ascites |
| Storage buffer: |
| Preservative:??no |
| Constituents:??PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity:??Anti-active Rab35 antibody recognizes active Rab35 proteins of vertebrates. |
| Storage Conditions:??Store at -20°C. Avoid freeze / thaw cycles |
| ? |
| Immunoprecipitation/Western blot:?? |
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Rab35 activation assay. Purified full-length Rab35 proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the anti-active Rab35 monoclonal antibody (Cat. No. 26922). Immunoblot was with an anti-Rab35 polyclonal antibody (Cat. No. 21078). |
| ? |
| Publications: |
| 1.??VASP promotes TGF-β activation of hepatic stellate cells by regulating Rab11 dependent plasma membrane targeting of TGF-β receptors ????Hepatology Volume 61, Issue 1, pages 361–374, January 2015 |
| 2.??Rab5 Activity Regulates GLUT4 Sorting into Insulin-Responsive and Non-Insulin-Responsive Endosomal Compartments: A Potential Mechanism for Development of Insulin Resistance ????Endocrinology. 2014 Sep;155(9):3315-28 |
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