脫氧核糖核酸酶 I
產(chǎn)品名稱： 脫氧核糖核酸酶 I
英文名稱： DNase I
產(chǎn)品編號： AMPD1-1KT
產(chǎn)品價格： 0
產(chǎn)品產(chǎn)地： 進(jìn)口
品牌商標(biāo)： Sigma-Aldrich
更新時間： null
使用范圍： null
默克生命科學(xué)
- 聯(lián)系人 : 默克
- 地址 : 上海市浦東新區(qū)東育路227弄3號前灘世貿(mào)中心(二期) C棟, 15-18層
- 郵編 : 200040
- 所在區(qū)域 : 上海
- 電話 : 400-960-9965轉(zhuǎn)2 點擊查看
- 傳真 : 點擊查看
- 郵箱 : 3538241589@qq.com;helen.jiang@merckgroup.com
- 二維碼 : 點擊查看
Deoxyribonuclease I (DNase I) is an endonuclease isolated from bovine pancreas that digests double- and single-stranded DNA into oligo- and mononucleotides. Using the Reaction Buffer provided, DNA is removed from RNA preparations in a 15 minute digestion at room temperature. The DNase I is then inactivated by heating with the Stop Solution. Heating also denatures hairpins in the RNA, so the RNA can be used directly in reverse transcription.
Many commercial DNase I formulations are contaminated with residual RNases. This RNase contamination can destroy or degrade valuable RNA samples prior to reverse transcription. Laboratory comparisons have shown that Sigma′s Amplification Grade DNase I demonstrates lower RNase activity than that from several leading molecular biology product suppliers.