Size??100ug
Cat code??200955
Species Cross-reactivity??H,M,R
Key Application??WB,ICC
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG1
Molecular Weight??42kDa
Sensitivity??This antibody detects endogenous levels of WDR77 and does not cross-react with related proteins.
Immunogen??Purified recombinant human WDR77 protein fragments expressed in E.coli.
UniProt Number??Q9BQA1
GeneBank ID??NM_024102
Subcellular location??Nucleus.Cytoplasm.
Formulation??Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Altername??2610312E17Rik;Androgen receptor cofactor p44;C79984;HKMT1069;MEP 50;MEP-50;MEP50;MEP50_HUMAN;Methylosome protein 50;MGC2722;Nbla10071;p44;p44/Mep50;RGD1310479;RP11 552M11.3;WD repeat containing protein 77;WD repeat domain 77;WD repeat-containing protein 77;WDR77.
Gene_symbol??p44;MEP50;MEP-50;HKMT1069;Nbla10071;p44/Mep50;RP11-552M11.3.
Summary??WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex,which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061).This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al.,2002 [PubMed 11756452]).[supplied by OMIM,Mar 2008]
UniPort summary  Function??Non-catalytic component of the 20S PRMT5-containing methyltransferase complex,which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones.This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles.Might play a role in transcription regulation.The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4),methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.
Application Images :??
Western blot detection of WDR77 antibody in C6,3T3 and K562 cell lysates using WDR77 antibody (1:1000 diluted).Predicted band size:42KDa.Observed band size:42KDa.
Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and then permeabilized in 0.1% Triton X-100，next using anti-WDR77 antibody (dilution 1:100).